Peptidoglycan biosynthesis by preparations from Bacillus licheniformis: cross-linking of newly synthesized chains to preformed cell wall.

The Biochemical journal ·Vol. 139 ·No. 3 ·1974-06-00 ·Pages 781-4

Ward JB, Perkins HR

Abstract

A wall-plus-membrane preparation from a Bacillus licheniformis mutant incorporated radioactivity from a peptidoglycan precursor in which the free amino group of diaminopimelic acid was blocked by (14)C-labelled acetyl group. This incorporation was penicillin-sensitive. The enzymically degraded product contained cross-linked dimers, showing that newly synthesized peptidoglycan chains had been cross-linked to the pre-existing cell wall.

MeSH Terms

Amino Acids, Diamino/metabolism Bacillus/metabolism Carbon Radioisotopes Cell Membrane Cell Wall/metabolism Chromatography, Gel Electrophoresis Mutation Penicillins/metabolism Peptidoglycan/biosynthesis Pimelic Acids/metabolism

Chemicals

Amino Acids, Diamino Carbon Radioisotopes Penicillins Peptidoglycan Pimelic Acids

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Ward J B

Perkins H R

References (9)

9 references, click to expand

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Biosynthesis of the peptidoglycan of bacterial cell walls. XI. Formation of the isoglutamine amide group in the cell walls of Staphylococcus aureus.
J Biol Chem. 1968 Feb 25;243(4):783-90 PMID: 5638594
The purification and properties of two staphylolytic enzymes from Streptomyces griseus.
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Autolytic enzymes in growth of bacteria.
Nature. 1971 Jan 22;229(5282):272-3 PMID: 4927203
Biosynthesis of peptidoglycan by a cell wall preparation of Staphylococcus aureus and its inhibition by penicillin.
Biochem Biophys Res Commun. 1972 Mar 10;46(5):1909-17 PMID: 4259358
Role of the penicillin-sensitive transpeptidation reaction in attachment of newly synthesized peptidoglycan to cell walls of Micrococcus luteus.
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3355-9 PMID: 4343965
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Biochem J. 1973 Dec;135(4):721-8 PMID: 4778270

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1390781

Published

1974-06-00

Pages

781-4

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1166343

Subset

External Links

PubMed source DOI: 10.1042/bj1390781 PMC Full Text View journal details

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Peptidoglycan biosynthesis by preparations from Bacillus licheniformis: cross-linking of newly synthesized chains to preformed cell wall.

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