Autolysis in Staphylococcus aureus: preferential release of old cell walls.

Journal of bacteriology ·Vol. 119 ·No. 3 ·1974-09-00 ·Pages 672-6

Gilpin RW, Narrod S, Wong W, Young FE, Chatterjee AN

Abstract

The kinetics of release of old versus new cell wall in two strains of Staphylococcus aureus were studied during autolysis. In both strains the autolytic enzyme is an amidase. Cells were double labeled with (3)H and (14)C, and the distribution of radioactivity in the cell walls was monitored during autolysis. In all cases the rate of release of steady-state lable from peptidoglycan was significantly higher than that of pulse label. Identical results were obtained with whole cells or isolated cell walls. The results suggest that in S. aureus the old cell wall is preferentially released during autolysis.

MeSH Terms

Amidohydrolases/metabolism Autolysis Bacteriolysis Carbon Radioisotopes Cell Wall/enzymology,metabolism Glycine/metabolism Peptidoglycan/metabolism Staphylococcus/enzymology,metabolism Teichoic Acids/metabolism Tritium

Chemicals

Carbon Radioisotopes Peptidoglycan Teichoic Acids Tritium Amidohydrolases Glycine

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Gilpin R W

Narrod S

Wong W

Young F E

Chatterjee A N

References (13)

13 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.119.3.672-676.1974

Published

1974-09-00

Pages

672-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC245667

Subset

External Links

PubMed source DOI: 10.1128/jb.119.3.672-676.1974 PMC Full Text View journal details

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Autolysis in Staphylococcus aureus: preferential release of old cell walls.

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