Physiology of sporeforming bacteria associated with insects: minimal nutritional requirements for growth, sporulation, and parasporal crystal formation of Bacillus thuringiensis.

Applied microbiology ·Vol. 28 ·No. 1 ·1974-07-00 ·Pages 124-8

Nickerson KW, Bulla LA

Abstract

A defined medium is described in which 18 strains of Bacillus thuringiensis representing the 12 established serotypes grow, sporulate, and produce a parasporal crystal. This minimal medium contains glucose and salts supplemented with either aspartate, glutamate, or citrate. These organic acids are required and cannot be replaced by vitamin mixtures or succinate even though succinate is taken up at a rate similar to that of aspartate, glutamate, and citrate.

MeSH Terms

Aspartic Acid/metabolism Bacillus/growth & development,metabolism Carbon Radioisotopes Citrates/metabolism Crystallography Culture Media Glutamates/metabolism Insect Control Pest Control, Biological Serotyping Spores, Bacterial/growth & development Succinates/metabolism

Chemicals

Carbon Radioisotopes Citrates Culture Media Glutamates Succinates Aspartic Acid

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Nickerson K W

Bulla L A

References (17)

17 references, click to expand

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Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.28.1.124-128.1974

Published

1974-07-00

Pages

124-8

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC186607

Subset

External Links

PubMed source DOI: 10.1128/am.28.1.124-128.1974 PMC Full Text

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Physiology of sporeforming bacteria associated with insects: minimal nutritional requirements for growth, sporulation, and parasporal crystal formation of Bacillus thuringiensis.

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