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PMID: 4829930 Published · ppublish English Journal Article

Purification and properties of undecyl acetate esterase from Pseudomonas cepacia grown on 2-tridecanone.

Journal of bacteriology ·Vol. 118 ·No. 3 ·1974-06-00 ·Pages 880-9

Shum AC, Markovetz AJ

Abstract

Undecyl acetate esterase has been purified from Pseudomonas cepacia grown on the methyl ketone, 2-tridecanone. The K(m) for undecyl acetate was 2.3 x 10(-2) M. Polyacrylamide gel electrophoresis indicated that two esterase bands were being recovered during purification. These bands were separated by preparative polyacrylamide gel electrophoresis. Molecular weights were estimated to be approximately 34,500 by several methods. Molecular sieve polyacrylamide gel electrophoresis indicated that the two esterases had the same molecular weight but different charge, which is indicative of isoenzymes.

MeSH Terms
Acetates/metabolism Ammonium Sulfate Carbon Radioisotopes Chemical Precipitation Chromatography, DEAE-Cellulose Chromatography, Gel Chromatography, Thin Layer Colorimetry Culture Media Electrophoresis, Polyacrylamide Gel Esterases/isolation & purification,metabolism Hydrogen-Ion Concentration Hydrolysis Ketones/metabolism Manometry Molecular Weight Pseudomonas/enzymology,growth & development,metabolism Temperature Tritium
Chemicals
Acetates Carbon Radioisotopes Culture Media Ketones Tritium Esterases Ammonium Sulfate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shum A C
Markovetz A J
References (31)
31 references, click to expand
  1. A comparison by the use of gel electrophoresis of soluble protein components and esterase enzymes of some group D Streptococci.
    J Gen Microbiol. 1965 Sep;40(3):413-9 PMID: 4159861
  2. Bacterial oxidation of 2-tridecanone to 1-undecanol.
    J Bacteriol. 1967 Feb;93(2):649-55 PMID: 6020567
  3. The aerobic pseudomonads: a taxonomic study.
    J Gen Microbiol. 1966 May;43(2):159-271 PMID: 5963505
  4. Some esterases of the pea (Pisum sativum L.).
    Biochim Biophys Acta. 1968 Mar 25;151(3):587-96 PMID: 4296397
  5. Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresis.
    Arch Biochem Biophys. 1968 Jul;126(1):155-64 PMID: 5671059
  6. Oxidative degradation of methyl ketones. II. Chemical pathway for degradation of 2-tridecanone by Pseudomonas multivorans and Pseudomonas aeruginosa.
    J Bacteriol. 1968 Oct;96(4):1055-64 PMID: 4971883
  7. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  8. An enzyme system for aliphatic methyl ketone oxidation.
    Biochem Biophys Res Commun. 1969 Sep 24;37(1):31-8 PMID: 4981344
  9. The solubilization and gel electrophoresis of membrane enzymes by use of detergents.
    Biochim Biophys Acta. 1970 Jan 6;196(1):29-34 PMID: 4312697
  10. Protein and esterase patterns of two formae speciales of Fusarium oxysporum.
    Phytopathology. 1969 Oct;59(10):1409-11 PMID: 5374242
  11. Subterminal oxidation of aliphatic hydrocarbons.
    J Bacteriol. 1970 Apr;102(1):281-2 PMID: 4985544
  12. Taxonomy of the aerobic pseudomonads: Pseudomonas cepacia, P. marginata, P. alliicola and P. caryophylli.
    J Gen Microbiol. 1970 Feb;60(2):199-214 PMID: 5488054
  13. Acylation of - and -chymotrypsins by p-nitrophenyl acetate. Enzyme-substrate complex formation and pH dependence.
    J Biol Chem. 1971 Oct 10;246(19):5907-13 PMID: 5116657
  14. Comparative zone electrophoresis of enzymes of Pseudomonas solanacearum and Pseudomonas cepacia.
    J Bacteriol. 1971 Nov;108(2):799-803 PMID: 5128336
  15. Heterogeneity, molecular weight interrelationships and developmental genetics of the esterase isoenzymes of the rainbow trout.
    Biochim Biophys Acta. 1972 Feb 28;258(2):455-65 PMID: 5010297
  16. Taxonomic application of isozyme patterns produced with disc electrophoresis of some myxomycetes, order Physarales.
    Mycologia. 1972 Jul-Aug;64(4):830-40 PMID: 4339547
  17. A study of the enzyme patterns of some oral filamentous bacteria by starch gel electrophoresis.
    Arch Oral Biol. 1972 Apr;17(4):691-700 PMID: 4505460
  18. Heterogeneity of esterases and cell types in rat liver.
    Biochim Biophys Acta. 1972 Sep 19;284(1):128-35 PMID: 5073757
  19. Effects of high concentrations of salt on the esterase activity of human carbonic anhydrase.
    FEBS Lett. 1972 Jul 15;24(1):85-8 PMID: 4628741
  20. Characterization of esterases produced by a ruminal bacterium identified as Butyrivibrio fibrisolvens.
    J Bacteriol. 1973 Mar;113(3):1170-6 PMID: 4734862
  21. Esterases in human leukocytes.
    J Histochem Cytochem. 1973 Jan;21(1):1-12 PMID: 4694536
  22. Isolation of two acetyl esterases from extracts of Bacillus subtilis.
    J Bacteriol. 1973 Jun;114(3):1184-92 PMID: 4197268
  23. The biology of methyl ketones.
    J Lipid Res. 1971 Jul;12(4):383-95 PMID: 4950559
  24. Specificity and induction of undecyl acetate esterase from Pseudomonas cepacia grown on 2-tridecanone.
    J Bacteriol. 1974 Jun;118(3):890-97 PMID: 4208413
  25. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  26. Serum esterases. I. Two types of esterase (A and B) hydrolysing p-nitrophenyl acetate, propionate and butyrate, and a method for their determination.
    Biochem J. 1953 Jan;53(1):110-7 PMID: 13032041
  27. The reaction of p-nitrophenyl esters with chymotrypsin and insulin.
    Biochem J. 1954 Feb;56(2):288-97 PMID: 13140189
  28. Studies on bacterial esterases. II. Paper-electrophoresis of the esterases of Pseudomonas aeruginosa.
    Antonie Van Leeuwenhoek. 1957;23(3-4):273-7 PMID: 13509633
  29. THE ULTRACENTRIFUGE: PROBLEMS AND PROSPECTS.
    Biochemistry. 1963 Sep-Oct;2:887-905 PMID: 14087378
  30. AN APPARATUS FOR PREPARATIVE TEMPERATURE-REGULATED POLYACRYLAMIDE GEL ELECTROPHORESIS.
    Anal Biochem. 1964 Nov;9:351-69 PMID: 14238529
  31. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.
    Ann N Y Acad Sci. 1964 Dec 28;121:404-27 PMID: 14240539
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.118.3.880-889.1974
Published
1974-06-00
Pages
880-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC246835
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.118.3.880-889.1974 PMC Full Text View journal details
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