Enzyme purification by hydrophobic chromatography: an alternative approach illustrated in the purification of aspartate transcarbamoylase from wheat germ.

The Biochemical journal ·Vol. 137 ·No. 1 ·1974-01-00 ·Pages 127-30

Yon RJ

Abstract

Two adsorbents containing similar numbers of hydrocarbon (C(10)) chains but different numbers of carboxyl groups were made by chemical modification of Sepharose. The use of these adsorbents to purify proteins, under conditions where hydrophobic adsorption is partly resisted by electrostatic repulsion, is illustrated in the purification of aspartate transcarbamoylase (EC 2.1.3.2) from wheat germ.

MeSH Terms

Adsorption Aspartate Carbamoyltransferase/isolation & purification Chromatography Electrophoresis, Polyacrylamide Gel Evaluation Studies as Topic Hydroxyapatites Methods Plants/enzymology Polysaccharides Spectrophotometry, Ultraviolet Triticum/enzymology

Chemicals

Hydroxyapatites Polysaccharides Aspartate Carbamoyltransferase

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Yon R J

References (11)

11 references, click to expand

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Wheat-germ aspartate transcarbamoylase. Kinetic behaviour suggesting an allosteric mechanism of regulation.
Biochem J. 1972 Jun;128(2):311-20 PMID: 4563642
Hydrocarbon-coated sepharoses. Use in the purification of glycogen phosphorylase.
Biochem Biophys Res Commun. 1972 Oct 17;49(2):383-90 PMID: 4640365
Protein binding by agarose carrying hydrophobic groups in conjunction with charges.
Biochem Biophys Res Commun. 1973 Feb 5;50(3):751-7 PMID: 4689075
Affinity chromatography of serum albumin with fatty acids immobilized on agarose.
J Biol Chem. 1973 Apr 10;248(7):2447-51 PMID: 4698226
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Proc Natl Acad Sci U S A. 1973 Mar;70(3):778-81 PMID: 4197628
Chromatography of plant aminoacyl-tRNA synthetases on omega-aminoalkyl sepharose columns.
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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1370127

Published

1974-01-00

Pages

127-30

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1166091

Subset

External Links

PubMed source DOI: 10.1042/bj1370127 PMC Full Text View journal details

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Enzyme purification by hydrophobic chromatography: an alternative approach illustrated in the purification of aspartate transcarbamoylase from wheat germ.

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