Mycoplasma contamination of cultured amniotic fluid cells: potential hazard to prenatal chromosomal diagnosis.

Science (New York, N.Y.) ·Vol. 184 ·No. 4135 ·1974-04-26 ·Pages 477-80

Schneider EL, Stanbridge EJ, Epstein CJ, Golbus M, Abbo-Halbasch G, Rodgers G

Abstract

Amniotic fluid cell cultures were screened for mycoplasma contamination. Mycoplasma RNA's were observed in more than half the cultures examined. Karyotypic analyses of these contaminated cell cultures revealed a significant increase in chromosomal aberrations. These studies emphasize the need for screening for mycoplasma in cultured amniotic cells.

MeSH Terms

Amniocentesis Amniotic Fluid/cytology,microbiology Cells, Cultured Chromosome Aberrations Electrophoresis, Polyacrylamide Gel Female Humans Karyotyping Mosaicism Mycoplasma/isolation & purification,metabolism Pregnancy RNA, Bacterial/isolation & purification Tritium Uracil/metabolism Uridine/metabolism

Chemicals

RNA, Bacterial Tritium Uracil Uridine

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Schneider E L

Stanbridge E J

Epstein C J

Golbus M

Abbo-Halbasch G

Rodgers G

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.184.4135.477

Published

1974-04-26

Pages

477-80

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.184.4135.477 View journal details

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Mycoplasma contamination of cultured amniotic fluid cells: potential hazard to prenatal chromosomal diagnosis.

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