Electron microscopy of Staphylococcus aureus cells and cell walls after treatment with lysozyme Chalaropsis.

Journal of bacteriology ·Vol. 117 ·No. 2 ·1974-02-00 ·Pages 900-3

Robinson JP, Robinson RD, Hash JH

Abstract

Lysozyme Chalaropsis, a beta-1,4-N,6-O-diacetylmuramidase, attacks the cell wall murein of Staphylococcus aureus H in a random manner. In hypertonic sucrose solutions, true protoplasts are produced and, in the process, mesosomal tubules are extruded into the medium.

MeSH Terms

Cell Wall/metabolism Hypertonic Solutions Microscopy, Electron Mitosporic Fungi/enzymology Muramidase/metabolism Peptidoglycan/metabolism Protoplasts Staphylococcus/cytology,metabolism Sucrose

Chemicals

Hypertonic Solutions Peptidoglycan Sucrose Muramidase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Robinson J P

Robinson R D

Hash J H

References (8)

8 references, click to expand

PURIFICATION AND PROPERTIES OF STAPHYLOLYTIC ENZYMES FROM CHALAROPSIS SP.
Arch Biochem Biophys. 1963 Sep;102:379-88 PMID: 14072516
Electron microscopy and viability of lysostaphin-induced staphylococcal spheroplasts, protoplast-like bodies, and protoplasts.
J Bacteriol. 1969 Jan;97(1):396-401 PMID: 5764340
ELECTRON MICROSCOPE STUDY OF THE RELATIONSHIP BETWEEN MESOSOME LOSS AND THE STABLE L STATE (OR PROTOPLAST STATE) IN BACILLUS SUBTILIS.
J Bacteriol. 1964 Aug;88:457-67 PMID: 14203364
The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.
J Cell Biol. 1963 Apr;17:208-12 PMID: 13986422
The isolation of protoplasts from Bacillus megaterium by controlled treatment with lysozyme.
J Bacteriol. 1953 Dec;66(6):688-95 PMID: 13117795
FORMATION OF "PROTOPLASTS" OF STAPHYLOCOCCUS AUREUS WITH A FUNGAL N-ACETYLHEXOSAMINIDASE.
J Bacteriol. 1964 Feb;87:432-7 PMID: 14151068
Electron microscopy during release and purification of mesosomal vesicles and protoplast membranes from Staphylococcus aureus.
J Bacteriol. 1971 Sep;107(3):907-17 PMID: 4106221
The N,O-diacetylmuramidase of Chalaropsis species. I. Purification and crystallization.
J Biol Chem. 1967 Dec 10;242(23):5586-90 PMID: 12325376

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.117.2.900-903.1974

Published

1974-02-00

Pages

900-3

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC285587

Subset

External Links

PubMed source DOI: 10.1128/jb.117.2.900-903.1974 PMC Full Text View journal details

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Electron microscopy of Staphylococcus aureus cells and cell walls after treatment with lysozyme Chalaropsis.

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