Home LiteratureArticle Details
PMID: 4797266 Published · ppublish English Journal Article

Isolation and preliminary characterization of the RNA-containing R-type, virus-like particle of BHK-21 cells.

Journal of virology ·Vol. 12 ·No. 5 ·1973-11-00 ·Pages 1164-72

Albu E, Holmes KV

Abstract

An R-type virus-like particle (VLP) has been isolated from the medium of BHK-21-F cells by ultracentrifugation and polyethylene glycol precipitation. The R-type VLP contains RNA which sediments at 60 to 70S in sucrose density gradients and has a molecular weight of approximately 10(7), as estimated by gel electrophoresis. The R-type VLP can be labeled with (3)H-uridine in the presence of actinomycin D. On the basis of morphology, site of maturation, and preliminary biochemical characterization, the R-type VLP does not appear to fit into any of the major groups of animal viruses.

MeSH Terms
Animals Carbon Radioisotopes Cell Line Cells, Cultured Centrifugation, Density Gradient Clone Cells Cricetinae Dactinomycin/pharmacology Electrophoresis, Polyacrylamide Gel Kidney Microscopy, Electron RNA Viruses/drug effects,growth & development,isolation & purification,metabolism RNA, Viral/isolation & purification Thymidine/metabolism Tritium Uridine/metabolism Virus Replication
Chemicals
Carbon Radioisotopes RNA, Viral Tritium Dactinomycin Thymidine Uridine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Albu E
Holmes K V
References (40)
40 references, click to expand
  1. Noninfectious RSV deficient in DNA polymerase.
    Virology. 1971 Jan;43(1):313-6 PMID: 5101018
  2. RNA-dependent DNA polymerase in virions of Rous sarcoma virus.
    Nature. 1970 Jun 27;226(5252):1211-3 PMID: 4316301
  3. Morphogenesis of avian infectious bronchitis virus and a related human virus (strain 229E).
    J Virol. 1967 Oct;1(5):1019-27 PMID: 5630226
  4. Electron microscopic observations on the association of viruses with membrane systems in hamster tumor cells propagated in tissue culture.
    J Natl Cancer Inst. 1969 Sep;43(3):653-69 PMID: 4309614
  5. Electron microscopic studies of coronavirus.
    J Gen Virol. 1971 Aug;12(2):161-8 PMID: 4107843
  6. A method for determining the sedimentation behavior of enzymes: application to protein mixtures.
    J Biol Chem. 1961 May;236:1372-9 PMID: 13767412
  7. AN ELECTRON MICROSCOPE STUDY OF THE DEVELOPMENT OF A MOUSE HEPATITIS VIRUS IN TISSUE CULTURE CELLS.
    J Cell Biol. 1965 Jan;24:57-78 PMID: 14286297
  8. SYRIAN HAMSTER FIBROBLAST CELL LINE BHK21 AND ITS DERIVATIVES.
    Nature. 1964 Sep 26;203:1355-7 PMID: 14207308
  9. Analysis of high-molecular-weight ribonucleic acid associated with intracisternal A particles.
    J Virol. 1973 Feb;11(2):287-98 PMID: 4688704
  10. Differential enhancement of R-type virus particles in polyoma-transformed BHK-21 cells by dimethyl sulfoxide.
    J Natl Cancer Inst. 1972 Sep;49(3):911-4 PMID: 4345972
  11. Isolation and characterization of extracellular virus-like particles from BHK cells. Brief report.
    Arch Gesamte Virusforsch. 1971;35(2):317-21 PMID: 4109498
  12. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.
    Biochemistry. 1968 Feb;7(2):668-74 PMID: 4868544
  13. Polyoma transformation of hamster cell clones--an investigation of genetic factors affecting cell competence.
    Virology. 1962 Feb;16:147-51 PMID: 14468055
  14. Deficiency of viral ribonucleic acid-dependent deoxyribonucleic acid polymerase in noninfectious virus-like particles released from murine sarcoma virus-transformed hamster cells.
    J Virol. 1972 Mar;9(3):488-93 PMID: 4335663
  15. Glycosphingolipids of plasma membranes of cultured cells and an enveloped virus (SV5) grown in these cells.
    Proc Natl Acad Sci U S A. 1970 May;66(1):57-64 PMID: 4320464
  16. Physical properties of Rous Sarcoma Virus RNA.
    Proc Natl Acad Sci U S A. 1968 Aug;60(4):1511-8 PMID: 4299948
  17. Plasma membrane lipids and parainfluenza virus assembly.
    Virology. 1970 Apr;40(4):939-47 PMID: 4317362
  18. Growth of rubella virus in BHK21 cells. II. Enhancing effect of DEAE-dextran, semicarbazide and low doses of metabolic inhibitors.
    Proc Soc Exp Biol Med. 1967 Aug-Sep;125(4):1092-8 PMID: 4167132
  19. The effects of cesarean derivation and foster nursing procedures on enzootic viruses of the LSH strain of inbred hamsters.
    Lab Anim Care. 1970 Apr;20(2):232-7 PMID: 4315778
  20. An electron microscopic study of moderate and virulent virus-cell interactions of the parainfluenza virus SV5.
    Virology. 1966 Nov;30(3):411-26 PMID: 4288418
  21. On the role of the response of the cell membrane in determining virus virulence. Contrasting effects of the parainfluenza virus SV5 in two cell types.
    J Exp Med. 1966 Sep 1;124(3):501-20 PMID: 4288512
  22. Comparison of immature (rapid harvest) and mature Rous sarcoma virus particles.
    Virology. 1972 Dec;50(3):851-64 PMID: 4344944
  23. Some structural and antigenic properties of intracisternal A particles occurring in mouse tumors (complement fixation-immunodiffusion-neuroblastoma-plasma-cell tumor).
    Proc Natl Acad Sci U S A. 1972 Jan;69(1):218-22 PMID: 4333040
  24. Significance of virus particles observed in spontaneous and induced tumors of the syrian hamster.
    Bibl Haematol. 1970;(36):559-65 PMID: 4376383
  25. Studies on the RNA from avian myeloblastosis virus.
    Virology. 1969 Jan;37(1):124-31 PMID: 4302800
  26. Physical and biochemical properties of progressive pneumonia virus.
    J Virol. 1971 Oct;8(4):573-8 PMID: 4108574
  27. Prevalence of type R virus-like particles in clones of BHK-21 cells.
    Virology. 1969 Aug;38(4):707-10 PMID: 5803950
  28. Lipids of plasma membranes of monkey and hamster kidney cells and of parainfluenza virions grown in these cells.
    Virology. 1969 Jun;38(2):255-68 PMID: 4306590
  29. Inhibition of high molecular weight RNA synthesis in a hamster melanoma by ethidium bromide in vivo.
    Hoppe Seylers Z Physiol Chem. 1972 Nov;353(11):1749-54 PMID: 4119283
  30. The nucleic acid of the Bryan strain of Rous sarcoma virus: purification of the virus and isolation of the nucleic acid.
    Proc Natl Acad Sci U S A. 1965 Jul;54(1):137-44 PMID: 4285422
  31. Synthesis of ribonucleic acid in baby-hamster kidney cells in the presence of actinomycin D.
    Biochem J. 1967 Dec;105(3):979-85 PMID: 16742574
  32. Virus particles in hamster tumors as revealed by electron microscopy.
    Proc Soc Exp Biol Med. 1966 Aug-Sep;122(4):1219-23 PMID: 4288219
  33. On the role of microtubules in movement and alignment of nuclei in virus-induced syncytia.
    J Cell Biol. 1968 Dec;39(3):526-43 PMID: 4301760
  34. Synthetic DNA-RNA hybrids and RNA-RNA duplexes as templates for the polymerases of the oncogenic RNA viruses.
    Nature. 1970 Oct 31;228(5270):430-2 PMID: 4320564
  35. Rabies vaccines: duration-of-immunity study in dogs.
    J Am Vet Med Assoc. 1971 Dec 1;159(11):1491-9 PMID: 5153870
  36. [Further observations on the virus of hamsters associated with BHK cells and with tumors].
    C R Acad Sci Hebd Seances Acad Sci D. 1968 May 13;266(20):2129-32 PMID: 4970684
  37. Absence of polymerase protein in virions of alpha-type rous sarcoma virus.
    Science. 1972 Sep 29;177(4055):1188-91 PMID: 4341568
  38. [Morphogenesis of a hamster virus associated with strain BHK or with tumors].
    C R Acad Sci Hebd Seances Acad Sci D. 1967 Jan 30;264(5):785-8 PMID: 4989212
  39. Transmission of a pigmented melanoma in golden hamsters by a cell-free ultrafiltrate.
    Nature. 1968 Aug 31;219(5157):979-80 PMID: 5673026
  40. Virus of avian erythromyeloblastic leukosis. IV. Sedimentation, density and hydration.
    Biochim Biophys Acta. 1954 May;14(1):12-7 PMID: 13160008
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.12.5.1164-1172.1973
Published
1973-11-00
Pages
1164-72
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356749
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.12.5.1164-1172.1973 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2