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PMID: 4795861 Published · ppublish English Journal Article

Intermittent DNA synthesis and periodic expression of enzyme activity in the cell cycle of WI-38.

The Journal of cell biology ·Vol. 58 ·No. 3 ·1973-09-00 ·Pages 564-73

Klevecz RR, Kapp LN

Abstract

Synchronous cultures of WI-38 were obtained using an automated system for detachment and partitioning of mitotic cells which operates without the use of inhibitors, altered medium, or lowered temperatures. The generation time in synchronous WI-38 is 19.5 h and the duration of S phase when determined from the percentage of labeled metaphase cells or nuclei is 12 h. DNA replication in WI-38 occurs in three temporally distinct and rapid bursts separated by intervals of greatly reduced synthesis within what is nominally described as the DNA synthetic (S) period. Lactate dehydrogenase (LDH) displayed maxima in G(1) between 2 and 4 h and again at 10 and 16 h. Peaks in LDH activity were coordinated with DNA replication in a fashion similar to that reported for diploid Chinese hamster cells. Oscillations in LDH activity are more pronounced in normal diploid fibroblasts than in established and neoplastic lines.

MeSH Terms
Animals Cell Division Cell Line Cricetinae DNA Replication Humans L-Lactate Dehydrogenase/metabolism Lung Spectrometry, Fluorescence Thymidine/metabolism Time Factors Tritium
Chemicals
Tritium L-Lactate Dehydrogenase Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Klevecz R R
Kapp L N
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41 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.58.3.564
Published
1973-09-00
Pages
564-73
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2109078
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.58.3.564 PMC Full Text View journal details
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