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PMID: 4776968 Published · ppublish English Journal Article

Intracellular forms of Adenovirus DNA. II. Isolation in dye-buoyant density gradients of a DNA-RNA complex from KB cells infected with Adenovirus type 2.

Journal of virology ·Vol. 12 ·No. 4 ·1973-10-00 ·Pages 793-807

Doerfler W, Lundholm U, Rensing U, Philipson L

Abstract

DNA-RNA complexes with different DNA/RNA ratios have been isolated from KB cells productively infected with human adenovirus type 2 by the dye-buoyant density procedure by using propidium iodide. Both the DNA and RNA components of these complexes are virus specific, and parental as well as newly synthesized viral DNA can be recovered from these complexes. The RNA component is susceptible to digestion with pancreatic ribonuclease at low and high salt concentrations. The RNA is in part liberated from the complex during purification over several cycles of equilibrium centrifugation in dye-buoyant density and Cs(2)SO(4) gradients. Analysis in the latter gradients reveals at least four classes of virus-specific nucleic acid: (i) RNA loosely bound and released during purification, (ii) and (iii) two distinct classes of RNA-DNA complexes with different DNA/RNA ratios, and (iv) DNA apparently not associated with RNA. The size of the RNA molecules is large but varying in the different classes of complexes. The biological function of these complexes is still uncertain, but they may be transcription complexes.

MeSH Terms
Adenoviridae/analysis Carbon Radioisotopes Carcinoma Cell Line Centrifugation, Density Gradient Chromatography, DEAE-Cellulose DNA, Viral/analysis,isolation & purification Dextrans Electrophoresis, Polyacrylamide Gel Humans Isotope Labeling Mouth Neoplasms Nucleic Acid Hybridization Nucleic Acids/isolation & purification Polyethylene Glycols RNA, Viral Uridine Viral Plaque Assay Virus Cultivation/instrumentation
Chemicals
Carbon Radioisotopes DNA, Viral Dextrans Nucleic Acids RNA, Viral Polyethylene Glycols Uridine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Doerfler W
Lundholm U
Rensing U
Philipson L
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31 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
Published
1973-10-00
Pages
793-807
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356697
Subset
IM
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