Abstract
This report describes the direct isolation and characterization of rickettsial ribosomes. Ribosomes from the rickettsia Coxiella burneti were isolated and partially characterized. The ribosomes had a sedimentation constant of about 70S and could be dissociated into 50 and 30S subunits. Electron microscopy revealed ribosomal particles with dimensions similar to those reported for other procaryotic organisms. Ribonucleic acid (RNA) species (23 and 16S) were isolated from the ribosomal particles. The nucleotide compositions of the ribosomal RNAs were found to be similar to those reported for bacterial ribosomal RNA. In addition to the high-molecular-weight ribosomal RNA, 5S RNA was also extracted from the organism.
MeSH Terms
Adenine/analysis
Autoradiography
Chromatography, Thin Layer
Coxiella/analysis,cytology
Cytosine/analysis
Electrophoresis, Polyacrylamide Gel
Guanine/analysis
Magnesium
Microscopy, Electron
Molecular Weight
RNA, Bacterial/analysis,isolation & purification
RNA, Ribosomal/analysis,isolation & purification
Ribosomes/analysis
Spectrophotometry, Ultraviolet
Ultracentrifugation
Uracil/analysis
Chemicals
RNA, Bacterial
RNA, Ribosomal
Uracil
Guanine
Cytosine
Magnesium
Adenine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Baca O G
Hersh R T
Paretsky D
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