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PMID: 4745423 Published · ppublish English Journal Article

Ribosomes and ribonucleic acids of Coxiella burneti.

Journal of bacteriology ·Vol. 116 ·No. 1 ·1973-10-00 ·Pages 441-6

Baca OG, Hersh RT, Paretsky D

Abstract

This report describes the direct isolation and characterization of rickettsial ribosomes. Ribosomes from the rickettsia Coxiella burneti were isolated and partially characterized. The ribosomes had a sedimentation constant of about 70S and could be dissociated into 50 and 30S subunits. Electron microscopy revealed ribosomal particles with dimensions similar to those reported for other procaryotic organisms. Ribonucleic acid (RNA) species (23 and 16S) were isolated from the ribosomal particles. The nucleotide compositions of the ribosomal RNAs were found to be similar to those reported for bacterial ribosomal RNA. In addition to the high-molecular-weight ribosomal RNA, 5S RNA was also extracted from the organism.

MeSH Terms
Adenine/analysis Autoradiography Chromatography, Thin Layer Coxiella/analysis,cytology Cytosine/analysis Electrophoresis, Polyacrylamide Gel Guanine/analysis Magnesium Microscopy, Electron Molecular Weight RNA, Bacterial/analysis,isolation & purification RNA, Ribosomal/analysis,isolation & purification Ribosomes/analysis Spectrophotometry, Ultraviolet Ultracentrifugation Uracil/analysis
Chemicals
RNA, Bacterial RNA, Ribosomal Uracil Guanine Cytosine Magnesium Adenine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Baca O G
Hersh R T
Paretsky D
References (16)
16 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1973-10-00
Pages
441-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC246440
Subset
IM
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