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PMID: 4738329 Published · ppublish English Journal Article

Nerve-muscle interaction in vitro. Role of acetylcholine.

The Journal of general physiology ·Vol. 62 ·No. 3 ·1973-09-00 ·Pages 255-70

Steinbach JH, Harris AJ, Patrick J, Schubert D, Heinemann S

Abstract

Nerve and muscle cells from clonal lines interact in vitro, resulting in the association on the muscle surface of an area of increased acetylcholine sensitivity with a site of nerve-muscle contact. This localization of acetylcholine sensitivity on the muscle cell to a site of contact between nerve and muscle was found to occur when acetylcholine receptors on the muscle had been blocked with alpha-neurotoxin. Localization was also found to occur when the nerve cell had been prevented from releasing acetylcholine. It is concluded that neither the presence of active acetylcholine receptors on the muscle, nor the release of acetylcholine from the nerve, was required for the events leading to the localization of acetylcholine sensitivity in vitro.

MeSH Terms
Acetylcholine/physiology Animals Clone Cells Iontophoresis Mice Muscles/drug effects,physiology Neurons/drug effects,physiology Pyridinium Compounds/pharmacology Rats Receptors, Cholinergic Toxins, Biological/pharmacology
Chemicals
Pyridinium Compounds Receptors, Cholinergic Toxins, Biological Acetylcholine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Steinbach J H
Harris A J
Patrick J
Schubert D
Heinemann S
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34 references, click to expand
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Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.62.3.255
Published
1973-09-00
Pages
255-70
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2226117
Subset
IM
External Links
PubMed source DOI: 10.1085/jgp.62.3.255 PMC Full Text View journal details
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