Synthesis of deoxyribonucleic acid in BHK-21-C13 cells.

The Biochemical journal ·Vol. 131 ·No. 3 ·1973-03-00 ·Pages 499-508

Hayton GJ, Pearson CK, Scaife JR, Keir HM

Abstract

Newly synthesized (3)H-labelled DNA was extracted from baby hamster kidney cells (BHK-21/C13 cells) and was shown to possess single-stranded properties when examined by column chromatography on benzoylated naphthoylated DEAE-cellulose, hydroxyapatite and methylated albumin on kieselguhr, and by its affinity for nitrocellulose filters. Some of the newly synthesized DNA was shown to be of lower molecular weight than the bulk of the DNA when examined by alkaline sucrose-density-gradient centrifugation. The properties observed were not affected by treatment of the DNA with ribonuclease, Pronase or amylase. The effect of the size of the DNA on its observed properties was examined and is discussed. It is concluded that DNA synthesis in BHK-21/C13 cells proceeds according to the discontinuous-mechanism model in at least one of the strands.

MeSH Terms

Animals Carbon Isotopes Cell Line Cellulose Centrifugation, Density Gradient Chemical Phenomena Chemistry Chromatography Chromatography, DEAE-Cellulose Cricetinae Culture Media DNA/biosynthesis DNA, Single-Stranded/biosynthesis Hydrogen-Ion Concentration Kidney/metabolism Nitro Compounds Nucleic Acid Denaturation Thymidine/metabolism Tritium Vibration

Chemicals

Carbon Isotopes Culture Media DNA, Single-Stranded Nitro Compounds Tritium Cellulose DNA Thymidine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Hayton G J

Pearson C K

Scaife J R

Keir H M

References (26)

26 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1310499

Published

1973-03-00

Pages

499-508

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1177497

Subset

External Links

PubMed source DOI: 10.1042/bj1310499 PMC Full Text View journal details

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Synthesis of deoxyribonucleic acid in BHK-21-C13 cells.

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