Home LiteratureArticle Details
PMID: 4734404 Published · ppublish English Journal Article

Formation and function of a complex of the C3 proactivator with a protein from cobra venom.

The Journal of experimental medicine ·Vol. 137 ·No. 2 ·1973-02-01 ·Pages 451-60

Cooper NR

Abstract

The role of C3 proactivator (C3PA) and a factor isolated from cobra venom (CoF) in the formation of a principle able to cleave C3 was investigated. The results clearly demonstrate two modes of interaction of C3PA with CoF. In isolated form, C3PA and CoF were found to form a reversible protein-protein complex in free solution. This complex had some C3 cleaving activity. In the presence of minute amounts of a partially purified normal serum substance, factor D, the C3PA-CoF complex was stabilized and its efficiency in cleaving C3 was greatly increased. Factor D is thus an activator of C3PA. A firm complex composed of C3PA and CoF, and possessing C3 cleaving activity, was also formed by addition of CoF to serum.

MeSH Terms
Animals Centrifugation, Density Gradient Complement System Proteins Electrophoresis, Polyacrylamide Gel Humans Iodine Isotopes Phagocytosis Snakes Venoms
Chemicals
Iodine Isotopes Venoms Complement System Proteins
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Cooper N R
References (21)
21 references, click to expand
  1. [Isolation and properties of a beta-glycoprotein from human serum].
    Clin Chim Acta. 1965 Oct;12(4):419-24 PMID: 5847757
  2. A method of trace iodination of proteins for immunologic studies.
    Int Arch Allergy Appl Immunol. 1966;29(2):185-9 PMID: 4160044
  3. A molecular concept of immune cytolysis.
    Arch Pathol. 1966 Sep;82(3):205-17 PMID: 5329367
  4. A new concept of immunosuppression in hypersensitivity reactions and in transplantation immunity.
    Surv Ophthalmol. 1966 Aug;11(4):498-505 PMID: 5919620
  5. Formation and functional significance of a molecular complex derived from the second and the fourth component of human complement.
    J Exp Med. 1967 Feb 1;125(2):359-80 PMID: 6019133
  6. Studies on the mode of action of the fifth, sixth and seventh component of human complement in immune haemolysis.
    Immunology. 1967 Jul;13(1):101-17 PMID: 4165629
  7. The derivation of two distinct anaphylatoxin activities from the third and fifth components of human complement.
    J Exp Med. 1968 Feb 1;127(2):371-86 PMID: 4383923
  8. Passive hemolysis by serum and cobra venom factor: a new mechanism inducing membrane damage by complement.
    Proc Natl Acad Sci U S A. 1969 Feb;62(2):521-7 PMID: 4978744
  9. Two anticomplementary factors in cobra venom: hemolysis of guinea pig erythrocytes by one of them.
    J Immunol. 1969 Nov;103(5):944-52 PMID: 4981797
  10. The biologic activities of guinea pig antibodies. II. Modes of complement interaction with gamma 1 and gamma 2-immunoglobulins.
    J Immunol. 1970 Feb;104(2):329-34 PMID: 4189670
  11. Isolation and properties of a glycine-rich beta-glycoprotein of human serum.
    Biochim Biophys Acta. 1970 Dec 22;221(3):529-35 PMID: 5499437
  12. The reaction mechanism of human C5 in immune hemolysis.
    J Exp Med. 1970 Oct 1;132(4):775-93 PMID: 5508377
  13. In vitro studies of complement function in sera of C4-deficient guinea pigs.
    J Exp Med. 1971 Jul 1;134(1):176-87 PMID: 4997584
  14. Functional relationship of factor B in the properdin system to C3 proactivator of human serum.
    J Immunol. 1971 Oct;107(4):1200-4 PMID: 5093780
  15. Isolation of the anticomplementary protein from cobra venom and its mode of action on C3.
    J Immunol. 1971 Dec;107(6):1666-72 PMID: 5120401
  16. Paroxysmal nocturnal hemoglobinuria. Hemolysis initiated by the C3 activator system.
    N Engl J Med. 1972 Jan 27;286(4):180-4 PMID: 5007123
  17. C3 proactivator convertase and its mode of action.
    J Exp Med. 1972 Apr 1;135(4):1003-8 PMID: 4111773
  18. Bactericidal and opsonic properties of C4-deficient guinea pig serum.
    J Immunol. 1972 Sep;109(3):477-86 PMID: 4403402
  19. The properdin system and immunity. I. Demonstration and isolation of a new serum protein, properdin, and its role in immune phenomena.
    Science. 1954 Aug 20;120(3112):279-85 PMID: 13186838
  20. THE MACROMOLECULAR NATURE OF THE FIRST COMPONENT OF HUMAN COMPLEMENT.
    J Exp Med. 1964 Apr 1;119:593-613 PMID: 14151101
  21. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.
    Ann N Y Acad Sci. 1964 Dec 28;121:404-27 PMID: 14240539
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.137.2.451
Published
1973-02-01
Pages
451-60
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2139485
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.137.2.451 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2