Glucocerebrosidase: reconstitution of activity from macromolecular components.

The Biochemical journal ·Vol. 131 ·No. 1 ·1973-01-00 ·Pages 173-6

Ho MW, O'Brien JS, Radin NS, Erickson JS

Abstract

Glucocerebrosidase activity was reconstituted in vitro from a soluble glycoprotein factor (P) and a particle-bound factor (C). The physiological significance of the system is discussed.

MeSH Terms

Carbon Isotopes Cerebrosides Chromatography, Thin Layer Coumarins Enzyme Activation Gaucher Disease/enzymology Glucosidases/metabolism Glycoproteins Humans Macromolecular Substances Spleen/enzymology

Chemicals

Carbon Isotopes Cerebrosides Coumarins Glycoproteins Macromolecular Substances Glucosidases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Ho M W

O'Brien J S

Radin N S

Erickson J S

References (9)

9 references, click to expand

Glycosidases in the nervous system. 3. Separation, purification, and substrate specificities of beta-galactosidases and beta-glucuronidase from brain.
J Biol Chem. 1968 Aug 25;243(16):4258-66 PMID: 5679961
Detection of the defect of Gaucher's disease and its carrier state in peripheral-blood leucocytes.
Lancet. 1970 Mar 21;1(7647):612-3 PMID: 4190558
METABOLISM OF GLUCOCEREBROSIDES. II. EVIDENCE OF AN ENZYMATIC DEFICIENCY IN GAUCHER'S DISEASE.
Biochem Biophys Res Commun. 1965 Jan 18;18:221-5 PMID: 14282020
IN VIVO CONVERSIONS OF CEREBROSIDE AND CERAMIDE IN RAT BRAIN.
J Lipid Res. 1965 Jan;6:140-5 PMID: 14280461
Gaucher's disease: deficiency of 'acid' -glucosidase and reconstitution of enzyme activity in vitro.
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2810-3 PMID: 5288260
Adult Gaucher's disease: kindred studies and demonstration of a deficiency of acid beta-glucosidase in cultured fibroblasts.
Am J Hum Genet. 1972 Jan;24(1):37-45 PMID: 5012691
Properties of cerebroside galactosidase.
Biochim Biophys Acta. 1968 May 1;152(3):599-610 PMID: 5656827
Ribonucleic acid synthesis by Escherichia coli C3000/L after infection by the ribonucleic acid coliphage ZIK/1, and properties of coliphage-ZIK/1 ribonucleic acid.
Biochem J. 1965 Oct;97(1):17-26 PMID: 16749099
A simple method for the isolation and purification of total lipides from animal tissues.
J Biol Chem. 1957 May;226(1):497-509 PMID: 13428781

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1310173

Published

1973-01-00

Pages

173-6

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1177451

Subset

External Links

PubMed source DOI: 10.1042/bj1310173 PMC Full Text View journal details

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Glucocerebrosidase: reconstitution of activity from macromolecular components.

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