Bacterial metabolism of 3-hydroxy-3-methylglutaric acid.

Journal of bacteriology ·Vol. 115 ·No. 1 ·1973-07-00 ·Pages 162-7

Ahmad N, Siddiqi M

Abstract

An organism belonging to Pseudomonadaceae and capable of utilizing 3-hydroxy-3-methylglutarate as sole carbon source has been isolated from soil. Whole-cell preparations catalyze the oxidation of acetoacetate, acetate, glyoxylate, and citric acid cycle intermediates. Cell-free extracts of 3-hydroxy-3-methylglutarate-grown cells show an adenosine triphosphate, coenzyme A (CoA), and Mg(2+)-dependent conversion of 3-hydroxy-3-methylglutarate to 3-hydroxy-3-methylglutaryl-CoA. Succinyl-CoA-generating system has no effect on the activation and catabolism of 3-hydroxy-3-methylglutarate.

MeSH Terms

Acetates/metabolism Acetoacetates/metabolism Adenosine Triphosphate/pharmacology Bacteria/enzymology,growth & development,metabolism Carbon Dioxide/biosynthesis Cell-Free System Chromatography, Paper Citrates/metabolism Coenzyme A/pharmacology Culture Media Glutarates/metabolism Glyoxylates/metabolism Magnesium/pharmacology Manometry Methylation Mevalonic Acid/metabolism Oxidation-Reduction Oxo-Acid-Lyases/metabolism Oxygen Consumption Soil Microbiology Succinates/metabolism Thiolester Hydrolases/metabolism

Chemicals

Acetates Acetoacetates Citrates Culture Media Glutarates Glyoxylates Succinates Carbon Dioxide Adenosine Triphosphate Thiolester Hydrolases Oxo-Acid-Lyases Magnesium Mevalonic Acid Coenzyme A

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Ahmad N

Siddiqi M

References (16)

16 references, click to expand

Bacterial metabolism of mevalonic acid.
J Bacteriol. 1967 Jan;93(1):207-14 PMID: 4289807
The reduction of beta-hydroxy-beta-methyl-glutaryl coenzyme A to mevalonic acid.
J Biol Chem. 1960 Sep;235:2572-8 PMID: 13818862
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Enzymatic synthesis of citric acid. II. Crystalline condensing enzyme.
J Biol Chem. 1951 Dec;193(2):691-702 PMID: 14907759
A simplified photometric method for the determination of citric acid in biological fluids.
J Biol Chem. 1952 Dec;199(2):531-6 PMID: 13022657
The effect of arsenate on aerobic phosphorylation.
J Biol Chem. 1953 Mar;201(1):235-43 PMID: 13044791
The acetate activating enzyme system of heart muscle.
J Biol Chem. 1953 Jul;203(1):35-45 PMID: 13069484
A colorimetric method for the estimation of acetoacetate.
Biochem J. 1954 Dec;58(4):699-704 PMID: 13230024
Biosynthesis of fatty acids in cell-free preparations. IV. Synthesis of fatty acids from acetate by a partially purified enzyme system from rabbit mammary gland.
Biochem J. 1957 Feb;65(2):348-63 PMID: 13403915
Isocitritase; enzyme properties and reaction equilibrium.
J Biol Chem. 1957 Nov;229(1):305-19 PMID: 13491582
[Methylglutaconase, a new hydrase participating in the metabolism of various carboxylic acids].
Biochem Z. 1958;329(6):476-89 PMID: 13535602
beta-Hydroxy-beta-methylglutaryl coenzyme A deacylase.
J Biol Chem. 1958 Aug;233(2):434-8 PMID: 13563516
The purification and properties of yeast isocitric lyase.
J Biol Chem. 1959 Jan;234(1):5-10 PMID: 13610881
THE ACTIVATION AND METABOLISM OF BETA-HYDROXY-BETA-METHYLGLUTARIC ACID.
J Biol Chem. 1964 Dec;239:4111-6 PMID: 14247656
The enzymatic cleavage of beta-hydroxy-beta-methylglutaryl coenzyme A to acetoacetate and acetyl coenzyme A.
J Biol Chem. 1955 Oct;216(2):727-36 PMID: 13271348
A modification of the nitroprusside method of analysis for glutathione.
Arch Biochem. 1951 Feb;30(2):217-25 PMID: 14811490

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.115.1.162-167.1973

Published

1973-07-00

Pages

162-7

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC246226

Subset

External Links

PubMed source DOI: 10.1128/jb.115.1.162-167.1973 PMC Full Text View journal details

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Bacterial metabolism of 3-hydroxy-3-methylglutaric acid.

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