Deoxyribonucleic acid synthesis in synchronously growing Mycoplasma gallisepticum.

Journal of bacteriology ·Vol. 115 ·No. 1 ·1973-07-00 ·Pages 117-20

Quinlan DC, Maniloff J

Abstract

Early log-phase cells of Mycoplasma gallisepticum A5969 were synchronized by holding in Eagle minimal essential medium (MEM) for 2 h. When transferred out of MEM into tryptose medium, the cells exhibited synchronous growth. Deoxyribonucleic acid (DNA) synthesis proceeded continuously during this growth but stopped during the period of cell division. One round of DNA replication was observed per cell doubling, and a unique region of DNA was found to be permanently bound to the membrane.

MeSH Terms

Cell Division Cell Membrane/metabolism Culture Media DNA Replication DNA, Bacterial/biosynthesis Deoxyribonucleases Mycoplasma/growth & development,metabolism Thymidine/metabolism Tritium

Chemicals

Culture Media DNA, Bacterial Tritium Deoxyribonucleases Thymidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Quinlan D C

Maniloff J

References (10)

10 references, click to expand

Regulation of deoxyribonucleic acid synthesis in Escherichia coli: dependence on growth rates.
Biochim Biophys Acta. 1966 Jun 22;119(3):517-25 PMID: 5336308
Analysis of the life cycle of Mycoplasma pneumoniae by synchronized division and by ultraviolet and x irradiations.
J Infect Dis. 1968 Feb;118(1):7-13 PMID: 4867945
Membrane attachment of the chromosome replication origin in Bacillus subtilis.
Cold Spring Harb Symp Quant Biol. 1968;33:695-705 PMID: 4977758
Evidence for stable attachment of DNA to membrane at the replication origin of Escherichia coli.
Biochem Biophys Res Commun. 1970 Oct 9;41(1):157-62 PMID: 4918012
Synchronized division of avian pleuropneumonialike organisms.
J Bacteriol. 1962 May;83:948-55 PMID: 14455057
A rapid method for synchronization of Staphylococcus aureus and Bacillus subtilis.
Biochem Biophys Res Commun. 1971 Sep;44(5):1125-30 PMID: 5003662
Membrane association of the deoxyribonucleic acid growing-point region in Mycoplasma gallisepticum.
J Bacteriol. 1972 Dec;112(3):1375-9 PMID: 4264449
Eagle's basal medium as a defined medium for Mycoplasma studies.
Microbios. 1972 Sep-Oct;6(22):179-85 PMID: 4571002
Thymine deficiency and the normal DNA replication cycle. I.
J Mol Biol. 1961 Apr;3:144-55 PMID: 13764647
The effect of hypotonic solutions on the morphology of cells of Mycoplasma gallisepticum.
Can J Microbiol. 1971 Sep;17(9):1203-5 PMID: 4107162

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.115.1.117-120.1973

Published

1973-07-00

Pages

117-20

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC246220

Subset

External Links

PubMed source DOI: 10.1128/jb.115.1.117-120.1973 PMC Full Text View journal details

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Deoxyribonucleic acid synthesis in synchronously growing Mycoplasma gallisepticum.

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