Isolation of streptolysin O by preparative polyacrylamide gel electrophoresis.

Infection and immunity ·Vol. 7 ·No. 3 ·1973-03-00 ·Pages 493-5

Van Epps DE, Andersen BR

Abstract

Streptolysin O giving a single band by Ouchterlony and acrylamide gel analysis has been isolated by ammonium sulfate precipitation, Sephadex gel filtration chromatography, and preparative polyacrylamide gel electrophoresis.

MeSH Terms

Chromatography Chromatography, Gel Electrophoresis, Polyacrylamide Gel Streptolysins/isolation & purification

Chemicals

Streptolysins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Van Epps D E

Andersen B R

References (6)

6 references, click to expand

Streptolysin O II. Relationship of Sulfyhdryl Groups to Activity.
Infect Immun. 1971 May;3(5):648-52 PMID: 16558031
Streptolysin O: sedimentation coefficient and molecular weight determinations.
J Bacteriol. 1969 Oct;100(1):526-7 PMID: 5344112
[A new method for the purification of streptolysin O].
C R Acad Sci Hebd Seances Acad Sci D. 1967 May 22;264(21):2524-5 PMID: 4964424
Purification of streptolysin O.
Nature. 1962 Oct 27;196:374-5 PMID: 14012262
The use of precipitin analysis in agar for the study of human streptococcal infections. III. The purification of some of the antigens detected by these methods.
J Exp Med. 1958 Sep 1;108(3):385-410 PMID: 13575674
Polyacrylamide gel electrophoresis.
Science. 1971 Apr 30;172(3982):440-51 PMID: 4927678

Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.7.3.493-495.1973

Published

1973-03-00

Pages

493-5

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC422706

Subset

External Links

PubMed source DOI: 10.1128/iai.7.3.493-495.1973 PMC Full Text View journal details

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Isolation of streptolysin O by preparative polyacrylamide gel electrophoresis.

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