Alkyl isocyanates as active-site-specific reagents for serine proteases. Identification of the active-site serine as the site of reaction.

Amino Acids/analysis Binding Sites Carbamates Carbon Isotopes Chromatography, Ion Exchange Chymotrypsin/analysis,antagonists & inhibitors Cyanates Endopeptidases Formates Hydrolysis Oxidation-Reduction Pancreatic Elastase/analysis,antagonists & inhibitors Peptides/analysis,isolation & purification Serine/analysis Subtilisins Trypsin

Amino Acids Carbamates Carbon Isotopes Cyanates Formates Peptides Serine Endopeptidases Subtilisins Chymotrypsin Pancreatic Elastase Trypsin