Collagen synthesis in vitro by embryonic spinal cord epithelium.

Science (New York, N.Y.) ·Vol. 179 ·No. 4070 ·1973-01-19 ·Pages 295-7

Trelstad RL, Kang AH, Cohen AM, Hay ED

Abstract

Isolated spinal cords from chick embryos (stages 12 to 15) were incubated in vitro with radioactive proline. The proteins synthesized were fractionated by coprecipitation with added carrier collagen, followed by molecularsieve and ion-exchange chromatography. A portion of the isotopically labeled proteins were found to be collagen molecules consisting only of alpha1 chains.

MeSH Terms

Amino Acids/analysis Animals Chick Embryo Chromatography Chromatography, Ion Exchange Collagen/analysis,biosynthesis Culture Techniques Epithelium/metabolism Hydroxyproline/analysis Nerve Tissue Proteins/analysis Proline/metabolism Spinal Cord/embryology,metabolism Tritium

Chemicals

Amino Acids Nerve Tissue Proteins Tritium Collagen Proline Hydroxyproline

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Trelstad R L

Kang A H

Cohen A M

Hay E D

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.179.4070.295

Published

1973-01-19

Pages

295-7

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.179.4070.295 View journal details

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Collagen synthesis in vitro by embryonic spinal cord epithelium.

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