Titration of antibodies to salmonella O antigens by enzyme-linked immunosorbent assay.

Infection and immunity ·Vol. 6 ·No. 5 ·1972-11-00 ·Pages 703-8

Carlsson HE, Lindberg AA, Hammarström S

Abstract

A new type of immunoassay (enzyme-linked immunosorbent assay, ELISA) has been used for the detection of antibodies against Salmonella O antigens. The results show that ELISA is specific and highly sensitive. When compared with the Widal reaction, passive hemagglutination, and quantitative precipitation, ELISA was shown to be more sensitive. In addition, immunoglobulin G and immunoglobulin M antibodies were both detected approximately as efficiently with ELISA.

MeSH Terms

Alkaline Phosphatase/pharmacology Animals Antibodies, Bacterial/analysis Antigens, Bacterial Chromatography, Gel Hemagglutination Tests Immune Sera Immunoassay Immunoglobulin G/analysis Immunoglobulin M/analysis Lipopolysaccharides/isolation & purification Polysaccharides, Bacterial/isolation & purification Precipitin Tests Rabbits/immunology Salmonella/immunology

Chemicals

Antibodies, Bacterial Antigens, Bacterial Immune Sera Immunoglobulin G Immunoglobulin M Lipopolysaccharides Polysaccharides, Bacterial Alkaline Phosphatase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Carlsson H E

Lindberg A A

Hammarström S

References (8)

8 references, click to expand

Enzyme-linked immunosorbent assay. II. Quantitative assay of protein antigen, immunoglobulin G, by means of enzyme-labelled antigen and antibody-coated tubes.
Biochim Biophys Acta. 1971 Dec 28;251(3):427-34 PMID: 11452886
Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies.
Immunochemistry. 1969 Jan;6(1):43-52 PMID: 4975324
Structural studies on the O-specific side-chains of the cell-wall lipopolysaccharide from Salmonella strasbourg.
Acta Chem Scand. 1970;24(4):1168-74 PMID: 5472638
Structural studies on the O-specific side-chains of the cell-wall lipopolysaccharide from Salmonella parathyphi A var. durazzo.
Acta Chem Scand. 1971;25(3):955-61 PMID: 5117491
Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.
Immunochemistry. 1971 Sep;8(9):871-4 PMID: 5135623
Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.
J Immunol. 1972 Jul;109(1):129-35 PMID: 4113792
Bacterial hemagglutination and hemolysis.
Bacteriol Rev. 1956 Sep;20(3):166-88 PMID: 13363771
IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS. XXX. CLEAVAGE OF A, B, AND H BLOOD-GROUP SUBSTANCES BY ALKALI.
Biochemistry. 1964 Jan;3:113-20 PMID: 14114492

Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.6.5.703-708.1972

Published

1972-11-00

Pages

703-8

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC422597

Subset

External Links

PubMed source DOI: 10.1128/iai.6.5.703-708.1972 PMC Full Text View journal details

Analysis Services

Titration of antibodies to salmonella O antigens by enzyme-linked immunosorbent assay.

Contact

WeChat Official Account