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PMID: 4633348 Published · ppublish English Journal Article

Transformation of germinated spores of Bacillus subtilis on agar plates.

Journal of bacteriology ·Vol. 114 ·No. 1 ·1973-04-00 ·Pages 445-7

Tanooka H

Abstract

l-Alanine-germinated spores of Bacillus subtilis developed a competence on agar plates after 10 h of incubation. Addition of marker amino acid to the plates was required for the transformation.

MeSH Terms
Agar Alanine/pharmacology Bacillus subtilis Carbon Isotopes Culture Media DNA Replication DNA, Bacterial/metabolism Spores, Bacterial Transformation, Genetic Tryptophan/pharmacology
Chemicals
Carbon Isotopes Culture Media DNA, Bacterial Tryptophan Agar Alanine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Tanooka H
References (7)
7 references, click to expand
  1. Recombination and integration in Bacillus subtilis transformation: involvement of DNA synthesis.
    J Mol Biol. 1965 Dec;14(2):534-57 PMID: 4956459
  2. Apparent dependence of transformation on the stage of deoxyribonucleic acid replication of recipient cells.
    Bacteriol Rev. 1968 Dec;32(4 Pt 1):291-6 PMID: 4974083
  3. Development of competence in thymine-starved Bacillus subtilis with chromosomes arrested at the terminus.
    J Bacteriol. 1969 Jan;97(1):166-73 PMID: 4974387
  4. Increase of thymidine, thymidylate and deoxycytidine kinase activites during germination of bacterial spores.
    Biochim Biophys Acta. 1971 Jan 1;228(1):26-37 PMID: 4993726
  5. Physiological and genetic factors affecting transformation of Bacillus subtilis.
    J Bacteriol. 1961 May;81:823-9 PMID: 13787458
  6. TRANSFORMABLE THYMINE-REQUIRING MUTANT OF BACILLUS SUBTILS.
    J Bacteriol. 1965 Jan;89:262-3 PMID: 14255676
  7. DEVELOPMENT OF COMPETENCE FOR TRANSFORMATION EXPERIMENTS IN AN OVERNIGHT CULTURE OF GERMINATING SPORES OF BACILLUS SUBTILIS.
    J Bacteriol. 1965 Aug;90:550-1 PMID: 14329474
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.114.1.445-447.1973
Published
1973-04-00
Pages
445-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC251785
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.114.1.445-447.1973 PMC Full Text View journal details
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