Selective replication of bacteriophage phi29 deoxyribonucleic acid in 6-(p-hydroxyphenylazo)-uracil-treated Bacillus subtilis.

Journal of virology ·Vol. 11 ·No. 1 ·1973-01-00 ·Pages 153-5

Schachtele CF, Reilly BE, De Sain CV, Whittington MO, Anderson DL

Abstract

Phage phi29 deoxyribonucleic acid (DNA) replicated under conditions where semiconservative DNA production in Bacillus subtilis host cells was blocked with 6-(p-hydroxyphenylazo)-uracil (HPUra). The time of initiation of phi29 DNA replication was not affected by HPUra, and normal quantities of viable phage were produced in the presence of the inhibitor. Studies with conditional lethal mutants of phage phi29 demonstrated the usefulness of HPUra for detection of viral-specific DNA production.

MeSH Terms

Azo Compounds/pharmacology Bacillus subtilis/drug effects,metabolism Bacteriophages/growth & development,metabolism DNA Replication DNA, Bacterial/biosynthesis DNA, Viral/biosynthesis Genes, Lethal Mitomycins/pharmacology Mutation Nucleic Acid Hybridization Thymidine/metabolism Time Factors Tritium Uracil Virus Replication

Chemicals

Azo Compounds DNA, Bacterial DNA, Viral Mitomycins Tritium Uracil Thymidine

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Schachtele C F

Reilly B E

De Sain C V

Whittington M O

Anderson D L

References (10)

10 references, click to expand

6-(p-hydroxyphenylazo)-uracil: a selective inhibitor of host DNA replication in phage-infected Bacillus subtilis.
Proc Natl Acad Sci U S A. 1970 Nov;67(3):1454-61 PMID: 4992015
Effect of elevated temperature on deoxyribonucleic acid synthesis in bacteriophage phi-29-infected Bacillus amyloliquefaciens.
J Virol. 1970 Oct;6(4):430-7 PMID: 5497888
Inhibition of bacterial DNA replication by 6-(p-hydroxyphenylazo)-uracil: differential effect on repair and semi-conservative synthesis in Bacillus subtilis.
J Mol Biol. 1971 Jul 14;59(1):1-16 PMID: 4997657
Control of DNA synthesis in Bacillus subtilis by phage phi e.
Virology. 1971 Apr;44(1):83-93 PMID: 4999126
Temperature-shift analysis of bacteriophage phi 29 gene expression in Bacillus amyloliquefaciens.
J Virol. 1971 Sep;8(3):352-4 PMID: 5119489
A genetic study of temperature-sensitive mutants of the Bacillus subtilis bacteriophage phi 29.
Virology. 1971 Mar;43(3):561-8 PMID: 5000908
Inhibition of bacterial DNA replication by 6-(p-hydroxy-phenylazo)-uracil.
Nat New Biol. 1972 May 17;237(72):72-4 PMID: 4402396
Transcription during the development of bacteriophage phi 29: production of host- and phi 29-specific ribonucleic acid.
J Virol. 1972 Dec;10(6):1170-8 PMID: 4630153
BACTERIOPHAGE DEOXYRIBONUCLEATE INFECTION OF COMPETENT BACILLUS SUBTILIS.
J Bacteriol. 1965 Mar;89:782-90 PMID: 14273661
Suppressor system in Bacillus subtilis 168.
J Bacteriol. 1969 Mar;97(3):1397-402 PMID: 4975748

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.11.1.153-155.1973

Published

1973-01-00

Pages

153-5

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC355073

Subset

External Links

PubMed source DOI: 10.1128/JVI.11.1.153-155.1973 PMC Full Text View journal details

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Selective replication of bacteriophage phi29 deoxyribonucleic acid in 6-(p-hydroxyphenylazo)-uracil-treated Bacillus subtilis.

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