Isolation of Vi antigen and a simple method for its measurement.

Applied microbiology ·Vol. 24 ·No. 4 ·1972-10-00 ·Pages 628-33

Wong KH, Feeley JC

Abstract

Vi antigen was purified from saline extract of Citrobacter strain 5396/38 by enzymatic digestion, concentration with ethanol, and precipitation with hexadecyltrimethylammonium bromide. The Vi antigen prepared by this method was 18 and 230 times more protective in mice than two other preparations isolated by an earlier method utilizing acid hydrolysis. A sensitive and specific method of electroimmunodiffusion was described for measurement of Vi antigen.

MeSH Terms

Animals Antigens, Bacterial/administration & dosage,analysis,isolation & purification Bacteriological Techniques Bromides Chemical Precipitation Deoxyribonucleases Enterobacteriaceae/immunology Enterobacteriaceae Infections/prevention & control Ethanol Immune Sera Immunodiffusion Injections, Intraperitoneal Injections, Subcutaneous Mice Pronase Quaternary Ammonium Compounds Rabbits/immunology Ribonucleases

Chemicals

Antigens, Bacterial Bromides Immune Sera Quaternary Ammonium Compounds Ethanol Deoxyribonucleases Ribonucleases Pronase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Wong K H

Feeley J C

References (17)

17 references, click to expand

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A simple method for the quantitation of submicrogram amounts of bacterial endotoxin.
Proc Soc Exp Biol Med. 1970 Mar;133(3):790-4 PMID: 5435570
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N Engl J Med. 1970 Sep 24;283(13):686-91 PMID: 4916913
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J Exp Med. 1961 May 1;113:921-34 PMID: 13724627
Immunological properties of Vi antigen isolated by chemical fractionation and by electrophoresis.
Tex Rep Biol Med. 1963;21:214-29 PMID: 13928496
Studies on Vi antigen. I. Relative Vi antigen content of V form cultures.
Proc Soc Exp Biol Med. 1952 May;80(1):55-8 PMID: 14930070
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Estimation of Vi antibody employing erythrocytes treated with purified Vi antigen.
Proc Soc Exp Biol Med. 1953 Apr;82(4):593-8 PMID: 13055942
Effect of a Vi-degrading enzyme on potency of typhoid vaccines in mice.
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Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.24.4.628-633.1972

Published

1972-10-00

Pages

628-33

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC380626

Subset

External Links

PubMed source DOI: 10.1128/am.24.4.628-633.1972 PMC Full Text

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