Mode of action of phleomycin on Bacillus subtilis.

Journal of bacteriology ·Vol. 111 ·No. 2 ·1972-08-00 ·Pages 586-92

Reiter H, Milewskiy M, Kelley P

Abstract

Phleomycin appears to act on the cell wall and membrane to induce the release of membrane-associated deoxyribonucleic acid (DNA) and the degradation of the DNA. Degradation occurs in a series of energy-requiring endonuclease and exonuclease reactions. These produce, first, single-strand breaks, then double-strand breaks, and finally almost complete solubilization of the cellular DNA. The in vivo inhibition of DNA synthesis by phleomycin is probably a secondary effect caused by the destruction of template DNA.

MeSH Terms

Antibiotics, Antineoplastic/pharmacology Bacillus subtilis/drug effects,enzymology,metabolism Bacterial Proteins/biosynthesis Carbon Isotopes Cell Membrane/drug effects Cell Wall/drug effects Centrifugation, Density Gradient DNA, Bacterial/antagonists & inhibitors,biosynthesis,metabolism Deoxyribonucleases Leucine/metabolism Nucleic Acid Denaturation Peptides/pharmacology RNA, Bacterial/antagonists & inhibitors,biosynthesis Solvents Templates, Genetic/drug effects Thymidine/metabolism Toluene Trichloroacetic Acid Tritium Uracil/metabolism

Chemicals

Antibiotics, Antineoplastic Bacterial Proteins Carbon Isotopes DNA, Bacterial Peptides RNA, Bacterial Solvents Tritium Toluene Uracil Trichloroacetic Acid Deoxyribonucleases Leucine Thymidine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Reiter H

Milewskiy M

Kelley P

References (14)

14 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.111.2.586-592.1972

Published

1972-08-00

Pages

586-92

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC251321

Subset

External Links

PubMed source DOI: 10.1128/jb.111.2.586-592.1972 PMC Full Text View journal details

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Mode of action of phleomycin on Bacillus subtilis.

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