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PMID: 4622898 Published · ppublish English Journal Article

Bacteriolytic enzymes produced by Myxococcus xanthus.

Journal of bacteriology ·Vol. 110 ·No. 1 ·1972-04-00 ·Pages 236-45

Sudo S, Dworkin M

Abstract

The bacteriolytic activities in the culture fluid of Myxococcus xanthus were purified and separated into six active fractions by the use of Bio-Gel CM-2 and Bio-Gel P-60. These fractions were identified as: (i) an amidase, (ii) a glucosaminidase, (iii) a glucosaminidase and an amidase, (iv) a protease with probable amidase activity, (v) another protease with probable amidase activity, and (vi) a peptidase active on both d-alanyl-diaminopimelate and d-alanyl-lysine peptide bonds. On one occasion, another amidase was eluted from Bio-Gel CM. Preliminary studies on some characteristics of the enzymes and their production during growth are reported.

MeSH Terms
Alanine/metabolism Amidohydrolases/isolation & purification Amino Acids Bacillus subtilis/cytology,metabolism Bacteria/enzymology,growth & development Bacteriolysis Carbon Isotopes Cell Wall/drug effects Chromatography, Gel Chromatography, Ion Exchange Endopeptidases/isolation & purification Glycoside Hydrolases/isolation & purification Hexosaminidases/isolation & purification Isotope Labeling Lysine Micrococcus/cytology,drug effects Peptide Hydrolases/isolation & purification Peptidoglycan Pimelic Acids Staphylococcus/cytology
Chemicals
Amino Acids Carbon Isotopes Peptidoglycan Pimelic Acids Glycoside Hydrolases Hexosaminidases Endopeptidases Peptide Hydrolases Amidohydrolases Lysine Alanine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sudo S
Dworkin M
References (20)
20 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.110.1.236-245.1972
Published
1972-04-00
Pages
236-45
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC247403
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.110.1.236-245.1972 PMC Full Text View journal details
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