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PMID: 4622819 Published · ppublish English Comparative Study Journal Article

Comparison of the immunofluorescent-cell counting and plaque methods for the assay of vaccinia virus.

Applied microbiology ·Vol. 23 ·No. 2 ·1972-02-00 ·Pages 214-7

Ibrahim AL, Loh PC

Abstract

The immunofluorescent-cell counting method was compared with the agarand liquid-overlay plaque techniques for the assay of vaccinia virus. In addition to being as quantitative, reproducible, and simple to use as the two plaque techniques, the immunofluorescent assay method was found to be more sensitive, rapid, and specific.

MeSH Terms
Agar Animals Antibody Formation Antigens, Viral/analysis Cell Count Cell Line Evaluation Studies as Topic Fluorescent Antibody Technique/standards Haplorhini HeLa Cells Hemolytic Plaque Technique Humans Immune Sera Kidney Methods Rabbits/immunology Vaccinia virus/growth & development,immunology,isolation & purification Virus Cultivation
Chemicals
Antigens, Viral Immune Sera Agar
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ibrahim A L
Loh P C
References (11)
11 references, click to expand
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    Appl Microbiol. 1965 Nov;13(6):865-71 PMID: 4286247
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  3. Enumeration of cell-infecting particles of Newcastle disease virus by the fluorescent antibody technique.
    J Exp Med. 1961 Feb 1;113:301-16 PMID: 13784769
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    Nature. 1962 Jan 13;193:150-1 PMID: 14480010
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    J Immunol. 1962 Jul;89:106-12 PMID: 13915711
  7. Human cytomegalovirus. Assay by counting infected cells.
    Virology. 1963 Apr;19:532-5 PMID: 13949074
  8. DEVELOPMENT OF VIRAL ANTIGEN AND INFECTIOUS VIRUS IN HELA CELLS INFECTED WITH REOVIRUS.
    J Immunol. 1963 Apr;90:548-53 PMID: 14082016
  9. A plaque technique for the titration of vaccinia virus in chick embryo cells and some features of vaccinial infection in this system.
    Virology. 1960 Apr;10:466-82 PMID: 14434422
  10. EFFECT OF P-FLUOROPHENYLALANINE ON THE SYNTHESIS OF VACCINIA VIRUS.
    Virology. 1965 Apr;25:560-74 PMID: 14329130
  11. THE RAPID DETECTION, TITRATION, AND DIFFERENTIATION OF VARIOLA AND VACCINIA VIRUSES BY A FLUORESCENT ANTIBODY-COVERSLIP CELL MONOLAYER SYSTEM.
    Virology. 1965 Apr;25:659-62 PMID: 14329139
Article Info
Journal
Applied microbiology
Abbr.
Appl Microbiol
ISSN
0003-6919
DOI
10.1128/am.23.2.214-217.1972
Published
1972-02-00
Pages
214-7
Language
English
Region
United States
NLM ID
7605802
PMCID
PMC380321
Subset
IM
External Links
PubMed source DOI: 10.1128/am.23.2.214-217.1972 PMC Full Text
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