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PMID: 4618481 Published · ppublish English Journal Article

Biogenesis of mitchondria. Phospholipid synthesis in vitro by yeast mitochondrial and microsomal fractions.

The Biochemical journal ·Vol. 144 ·No. 2 ·1974-11-00 ·Pages 265-75

Cobon GS, Crowfoot PD, Linnane AW

Abstract

The ability in vitro of yeast mitochondrial and microsomal fractions to synthesize lipid de novo was measured. The major phospholipids synthesized from sn-[2-(3)H]glycerol 3-phosphate by the two microsomal fractions were phosphatidylserine, phosphatidylinositol and phosphatidic acid. The mitochondrial fraction, which had a higher specific activity for total glycerolipid synthesis, synthesized phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine and phosphatidic acid, together with smaller amounts of neutral lipids and diphosphatidylglycerol. Phosphatidylcholine synthesis from both S-adenosyl[Me-(14)C]methionine and CDP-[Me-(14)C]choline appeared to be localized in the microsomal fraction.

MeSH Terms
Chromatography, Thin Layer Cytidine Diphosphate Choline/metabolism Glycerophosphates/metabolism In Vitro Techniques Microsomes/metabolism Mitochondria/metabolism Phosphatidic Acids/biosynthesis Phosphatidylcholines/biosynthesis Phosphatidylethanolamines/biosynthesis Phosphatidylinositols/biosynthesis Phosphatidylserines/biosynthesis Phospholipids/analysis,biosynthesis S-Adenosylmethionine/metabolism Saccharomyces cerevisiae/metabolism Subcellular Fractions
Chemicals
Glycerophosphates Phosphatidic Acids Phosphatidylcholines Phosphatidylethanolamines Phosphatidylinositols Phosphatidylserines Phospholipids Cytidine Diphosphate Choline S-Adenosylmethionine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cobon G S
Crowfoot P D
Linnane A W
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30 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1440265
Published
1974-11-00
Pages
265-75
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1168493
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1440265 PMC Full Text View journal details
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