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PMID: 4616874 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Ultraviolet mutagenesis and its repair in an Escherichia coli strain containing a nonsense codon.

Genetics ·Vol. 78 ·No. 4 ·1974-12-00 ·Pages 1035-49

Person S, McCloskey JA, Snipes W, Bockrath RC

Abstract

Ultraviolet mutagenesis and its repair were studied mainly in WU36-10-89, a uvr(-) strain of Escherichia coli containing a UAG mutation in a gene for leucine biosynthesis. Following ultraviolet (UV) irradiation revertants appearing with or without direct photoreactivation (PR) were classified according to the presence and type of suppressor they contained. We find UV mutation production to be quite specific. An analysis of revertants produced by UV indicates they are formed mainly from GC --> AT and that the miscoding is due to a cytosine residue at the site of mutation in a cytosine-thymine (CT) dimer. We propose that the dimer serves as template during some aspects of repair replication and at the time of replication the C in the dimer directs the insertion of A in the complementary strand. We also note that C --> A and T -->G changes caused by a CT dimer occur much less frequently.

MeSH Terms
Base Sequence Codon DNA Repair DNA Replication DNA, Bacterial Escherichia coli/metabolism,radiation effects Genes Genetic Code Leucine/metabolism Mutation Peptide Chain Termination, Translational Radiation Genetics Templates, Genetic Ultraviolet Rays
Chemicals
Codon DNA, Bacterial Leucine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Person S
McCloskey J A
Snipes W
Bockrath R C
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21 references, click to expand
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/78.4.1035
Published
1974-12-00
Pages
1035-49
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1213237
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/78.4.1035 PMC Full Text View journal details
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