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PMID: 4616225 Published · ppublish English Journal Article

Replication of colicinogenic factor E 1 DNA: evidence for a discontinuous replication mechanism.

Nucleic acids research ·Vol. 1 ·No. 9 ·1974-09-00 ·Pages 1153-64

Staudenbauer WL

Abstract

The mechanism of Col E 1 DNA replication was investigated in a plasmolysed cell system prepared from chloramphenicoltreated E. coli JC 411 (Col E 1). After pulse-labelling with (3)H-dTTP a considerable fraction of the newly synthesized DNA was recovered as single-stranded fragments. Upon alkali denaturation the pulse label was found in DNA chains sedimenting slower than unit length Col E 1 strands with a prominent peak at 5 S. During a chase with unlabeled precursors the label is transferred nearly completely into supercoiled Col E 1 DNA. DNA ligase appears to be required for the joining of the 5 S pieces since in the absence of NAD an accumulation of short fragments is observed.

MeSH Terms
Bacterial Proteins/biosynthesis Centrifugation, Density Gradient Chromatography, Affinity Colicins/biosynthesis DNA Replication DNA, Bacterial/isolation & purification DNA, Single-Stranded/biosynthesis,isolation & purification Escherichia coli/metabolism Nucleic Acid Conformation Protein Biosynthesis Thymine/metabolism Transcription, Genetic
Chemicals
Bacterial Proteins Colicins DNA, Bacterial DNA, Single-Stranded Thymine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Staudenbauer W L
References (19)
19 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/1.9.1153
Published
1974-09-00
Pages
1153-64
Language
English
Region
England
NLM ID
0411011
PMCID
PMC344337
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/1.9.1153 PMC Full Text View journal details
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