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PMID: 4615709 Published · ppublish English Journal Article

Radioautographic localization of labelled proteins after incubation of guinea-pig islets of Langerhans with (3H)tryptophan.

The Biochemical journal ·Vol. 140 ·No. 1 ·1974-04-00 ·Pages 22-3

Howell SL, Hellerström C, Whitfield M

Abstract

An analysis was made by electron-microscopic radioautography of the distribution of silver grains over storage granules of A(2) and B cells after incubation of isolated guinea-pig islets of Langerhans for 17h in the presence of [(3)H]tryptophan. A significant concentration of labelled proteins was present in the A(2) cell, but not in the B-cell granules.

MeSH Terms
Animals Guinea Pigs Islets of Langerhans/metabolism,ultrastructure Methods Microscopy, Electron Protein Biosynthesis Tritium Tryptophan/metabolism
Chemicals
Tritium Tryptophan
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Howell S L
Hellerström C
Whitfield M
References (7)
7 references, click to expand
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    Physiol Rev. 1971 Jan;51(1):158-200 PMID: 4924033
  3. A RELIABLE AND EASILY SECTIONED EPOXY EMBEDDING MEDIUM.
    Anat Rec. 1964 Oct;150:129-39 PMID: 14223588
  4. Amino acid metabolism in mammalian cell cultures.
    Science. 1959 Aug 21;130(3373):432-7 PMID: 13675766
  5. An investigation of glucagon biosynthesis in isolated pancreatic islets of guinea pigs.
    FEBS Lett. 1972 Oct 15;27(1):97-101 PMID: 11946816
  6. Biosynthesis of glucagon in isolated pancreatic islets of guinea pigs.
    Biochem J. 1974 Apr;140(1):13-21 PMID: 4615708
  7. Synthesis and secretion of growth hormone in the rat anterior pituitary. I. The intracellular pathway, its time course and energy requirements.
    J Cell Sci. 1973 Jan;12(1):1-21 PMID: 4266075
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1400022
Published
1974-04-00
Pages
22-3
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1167966
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1400022 PMC Full Text View journal details
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