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PMID: 4611919 Published · ppublish English Journal Article

Protection against gram-negative infections with antiserum to lipid A from Salmonella minnesota R595.

Infection and immunity ·Vol. 10 ·No. 6 ·1974-12-00 ·Pages 1195-201

Mullan NA, Newsome PM, Cunnington PG, Palmer GH, Wilson ME

Abstract

The ability of antisera to lipid A, induced in rabbits by immunization with lipid A complexed to various carriers, to protect mice against gram-negative infection and to inhibit the fluid loss caused by an enteropathogenic strain of Escherichia coli in the piglet ligated gut was investigated. No significant protection was obtained in either case, although passive hemolysis and quantitative precipitation tests showed the presence of antilipid A antibodies in the sera. Fluorescent antibody studies suggest that the lipid A is in a cryptic position on the surface of smooth strains of gram-negative bacteria.

MeSH Terms
Animals Antibodies, Bacterial/analysis Cattle/immunology Escherichia coli Infections/immunology Fluorescent Antibody Technique Goats/immunology Hemagglutination Tests Hemocyanins Hemolysis Histones Immune Sera/administration & dosage Immunity, Maternally-Acquired Immunization Schedule Injections, Intravenous Intestine, Small/immunology Intestines/immunology Klebsiella/immunology Lethal Dose 50 Lipids Male Mercaptoethanol Mice Precipitin Tests Rabbits/immunology Salmonella/immunology Salmonella Infections/immunology Serum Albumin, Bovine Swine
Chemicals
Antibodies, Bacterial Histones Immune Sera Lipids Serum Albumin, Bovine Mercaptoethanol Hemocyanins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Mullan N A
Newsome P M
Cunnington P G
Palmer G H
Wilson M E
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21 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.10.6.1195-1201.1974
Published
1974-12-00
Pages
1195-201
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC423086
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.10.6.1195-1201.1974 PMC Full Text View journal details
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