Home LiteratureArticle Details
PMID: 4607764 Published · ppublish English Journal Article

Selection procedure for mutants defective in the beta-methylgalactoside transport system of Escherichia coli utilizing the compound 2R-glyceryl-beta-D-galactopyranoside.

Journal of bacteriology ·Vol. 120 ·No. 1 ·1974-10-00 ·Pages 424-32

Silhavy TJ, Boos W

Abstract

A procedure has been devised that allows selection of mutants defective in the beta-methylgalactoside transport system (mgl) of Escherichia coli. This procedure utilizes the compound 2R-glyceryl-beta-d-galactopyranoside (glycerylgalactoside), which is known to be transported by only two transport system in E. coli, namely, the lactose and the beta-methylgalactoside transport systems. Mutants lacking glycerol-3-phosphate dehydrogenase (glpD) are sensitive to glycerol. Similarly, mutants lacking uridine diphosphate-galactose-4-epimerase (galE) are sensitive to galactose. Glycerylgalactoside is an inducer of the lactose operon and also a substrate for beta-galactosidase. Thus, a mgl(+)glpD galE lacY strain will not grow in the presence of glycerylgalactoside owing to accumulated glycerol-3-phosphate, galactose-1-phosphate, and uridine diphosphate-galactose. We have constructed such a strain and shown that mgl mutants can be obtained by selecting for those that grow in the presence of glycerylgalactoside.

MeSH Terms
Autoradiography Biological Transport, Active Carbohydrate Epimerases/biosynthesis Carbon Radioisotopes Chromatography, Paper Escherichia coli/enzymology,isolation & purification,metabolism Galactose/metabolism Galactosidases/biosynthesis Glycerol/metabolism Glycerolphosphate Dehydrogenase/biosynthesis Glycosides/metabolism Kinetics Methylgalactosides/metabolism Methylglycosides/metabolism Mutagens Mutation Nitrosoguanidines Succinates/metabolism Transduction, Genetic
Chemicals
Carbon Radioisotopes Glycosides Methylgalactosides Methylglycosides Mutagens Nitrosoguanidines Succinates Glycerolphosphate Dehydrogenase Galactosidases Carbohydrate Epimerases Glycerol Galactose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Silhavy T J
Boos W
References (27)
27 references, click to expand
  1. Multiple transport components for dicarboxylic amino acids in Streptococcus faecalis.
    J Biol Chem. 1970 Oct 25;245(20):5261-72 PMID: 4990299
  2. Structurally defective galactose-binding protein isolated from a mutant negative in the -methylgalactoside transport system of Escherichia coli.
    J Biol Chem. 1972 Sep 10;247(17):5414-24 PMID: 4626720
  3. Isolation and complementation of mutants in galactose taxis and transport.
    J Bacteriol. 1974 Feb;117(2):509-16 PMID: 4359647
  4. Role of the galactose transport system in the retention of intracellular galactose in Escherichia coli.
    J Mol Biol. 1969 Apr 14;41(1):109-20 PMID: 4896014
  5. The metabolic basis for masking of receptor-sites on E. coli K-12 for C21, a lipopolysaccharide core-specific phage.
    Arch Biochem Biophys. 1968 Oct;128(1):95-105 PMID: 4877876
  6. Segregation of New Lysogenic Types during Growth of a Doubly Lysogenic Strain Derived from Escherichia Coli K12.
    Genetics. 1954 Jul;39(4):440-52 PMID: 17247495
  7. HEREDITARY DEFECTS IN GALACTOSE METABOLISM IN ESCHERICHIA COLI MUTANTS, I. DETERMINATION OF ENZYME ACTIVITIES.
    Proc Natl Acad Sci U S A. 1959 Dec;45(12):1776-86 PMID: 16590575
  8. Properties of the glutamate transport system in Escherichia coli.
    J Bacteriol. 1967 Mar;93(3):1009-16 PMID: 5337827
  9. Genetic control of L-alpha-glycerophosphate system in Escherichia coli.
    J Mol Biol. 1968 Feb 14;31(3):371-87 PMID: 4866330
  10. A convenient synthesis of (2R)-glyceryl-beta-D-galactopyranoside. A substrate for beta-galactosidase, the lactose repressor, the galactose-binding protein, and the beta-methylgalactoside transport system.
    J Biol Chem. 1973 Sep 25;248(18):6571-4 PMID: 4581106
  11. The kinetics of L-aspartate transport in Neurospora crassa conidia.
    Biochim Biophys Acta. 1972 Dec 1;290(1):355-67 PMID: 4264473
  12. The periplasmic galactose binding protein of Escherichia coli.
    Science. 1971 Nov 5;174(4009):557-65 PMID: 4940020
  13. Isolation and properties of mutants of Escherichia coli with increased phosphorylations of thiomethyl-beta-galactoside.
    Biochim Biophys Acta. 1969;193(2):294-307 PMID: 4900754
  14. Close linkage between a galactose binding protein and the beta-methylgalactoside permease in Escherichia coli.
    Eur J Biochem. 1970 Apr;13(3):526-33 PMID: 4910389
  15. [ROLE OF LACTOSE AND ITS METABOLIC PRODUCTS IN THE INDUCTION OF THE LACTOSE OPERON IN ESCHERICHIA COLI].
    Biochim Biophys Acta. 1965 Apr 19;95:634-9 PMID: 14324815
  16. Formation of protoplasts' in mutant strains of Salmonella induced by galactose.
    Nature. 1959 Apr 18;183(4668):1131-2 PMID: 13657029
  17. Transduction of linked genetic characters of the host by bacteriophage P1.
    Virology. 1955 Jul;1(2):190-206 PMID: 13267987
  18. Agar layer method for production of high titer phage stocks.
    Proc Soc Exp Biol Med. 1951 Nov;78(2):372-5 PMID: 14911888
  19. Galactose-sensitive mutants of Salmonella.
    Nature. 1959 Oct 10;184(Suppl 15):1168-9 PMID: 13825592
  20. HEREDITARY DEFECTS IN GALACTOSE METABOLISM IN ESCHERICHIA COLI MUTANTS, II. GALACTOSE-INDUCED SENSITIVITY.
    Proc Natl Acad Sci U S A. 1959 Dec;45(12):1786-91 PMID: 16590576
  21. Isolation of the lac repressor.
    Proc Natl Acad Sci U S A. 1966 Dec;56(6):1891-8 PMID: 16591435
  22. Transport systems for galactose and galactosides in Escherichia coli. II. Substrate and inducer specificities.
    J Mol Biol. 1968 Sep 14;36(2):247-60 PMID: 4939625
  23. Growth stasis by accumulated L-alpha-glycerophosphate in Escherichia coli.
    J Bacteriol. 1965 Nov;90(5):1325-9 PMID: 5321485
  24. Pathway of fructose utilization by Escherichia coli.
    FEBS Lett. 1971 Feb 19;13(2):127-130 PMID: 11945648
  25. The regulation of the beta-methylgalactoside transport system and of the galactose binding protein of Escherichia coli K12.
    Eur J Biochem. 1971 Apr 30;19(4):457-70 PMID: 4996112
  26. Studies with alpha-ketoglutarate dehydrogenase mutants of Escherichia coli.
    Mol Gen Genet. 1969 Oct 13;105(2):182-90 PMID: 4904515
  27. The regulation and properties of the galactose transport system in Escherichia coli K12.
    J Biol Chem. 1974 Jan 25;249(2):553-8 PMID: 4588568
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.120.1.424-432.1974
Published
1974-10-00
Pages
424-32
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC245779
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.120.1.424-432.1974 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2