Renaturation of Escherichia coli tryptophanase after exposure to 8 M urea. Evidence for the existence of nucleation centers.

Apoproteins/isolation & purification Binding Sites Biochemical Phenomena Biochemistry Carboxy-Lyases/analysis Centrifugation, Density Gradient Dialysis Enzyme Activation/drug effects Escherichia coli/enzymology Ligases Osmolar Concentration Protein Conformation Protein Denaturation Pyridoxal Phosphate/pharmacology Serum Albumin/pharmacology Temperature Time Factors Tryptophanase/metabolism Ultracentrifugation Urea

Apoproteins Serum Albumin Pyridoxal Phosphate Urea Carboxy-Lyases Tryptophanase Ligases