Terminal cross-linking of DNA catalyzed by an enzyme system containing DNA ligase, DNA polymerase, and exonuclease of bacteriophage T7.

Base Sequence Carbon Radioisotopes Centrifugation, Density Gradient Chromatography, DEAE-Cellulose Chromatography, Ion Exchange Coliphages/enzymology DNA Nucleotidyltransferases/metabolism DNA Replication DNA, Viral/biosynthesis Escherichia coli/enzymology Exonucleases/metabolism Genes Mutation Nucleic Acid Conformation Polynucleotide Ligases/metabolism Species Specificity Spectrophotometry, Ultraviolet Thymidine/metabolism Thymine/metabolism Tritium Ultrasonics

Carbon Radioisotopes DNA, Viral Tritium DNA Nucleotidyltransferases Exonucleases Polynucleotide Ligases Thymine Thymidine