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PMID: 4598307 Published · ppublish English Journal Article

Isolation and function of the gene A initiator of bacteriophage phi-chi 174, a highly specific DNA endonuclease.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 4 ·1974-04-00 ·Pages 1549-53

Henry TJ, Knippers R

Abstract

The gene A product of Escherichia coli bacteriophage varphiX 174, necessary for initiation of varphiX DNA synthesis, has been identified and purified to near homogeneity. The gene A protein is shown to be a highly specific DNA endonuclease which breaks one phosphodiester bond in the viral strand of either double-stranded, circular varphiX replicative form I (RFI), or in single-stranded, circular varphiX viral DNA; it is inactive against all other species of DNA tested.

MeSH Terms
Carbon Radioisotopes Chromatography, DEAE-Cellulose Coliphages/analysis,enzymology,metabolism DNA Replication DNA Viruses/analysis,enzymology DNA, Circular/analysis DNA, Single-Stranded/analysis DNA, Viral/analysis,biosynthesis Deoxyribonucleases/biosynthesis,isolation & purification,metabolism Electrophoresis, Polyacrylamide Gel Escherichia coli Genes Genetic Code Viral Proteins/biosynthesis,isolation & purification,metabolism Virus Replication
Chemicals
Carbon Radioisotopes DNA, Circular DNA, Single-Stranded DNA, Viral Viral Proteins Deoxyribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Henry T J
Knippers R
References (46)
46 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.4.1549
Published
1974-04-00
Pages
1549-53
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388268
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.4.1549 PMC Full Text View journal details
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