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PMID: 4591944 Published · ppublish English Journal Article

Sodium-stimulated glutamate transport in osmotically shocked cells and membrane vesicles of Escherichia coli.

Journal of bacteriology ·Vol. 117 ·No. 3 ·1974-03-00 ·Pages 1093-8

Miner KM, Frank L

Abstract

Three phenotypically distinct strains of Escherichia coli B were studied: one in which the transport of glutamate was strongly stimulated by sodium, one in which the transport was relatively independent of sodium, and one which did not transport glutamate. Membrane vesicle preparations from the three strains followed the behavior of whole cells with respect to sodium-stimulated transport. Although glutamate-binding material could be released from cells by osmotic shock, its affinity for glutamate was not significantly influenced by sodium. Furthermore, the shocked cells retained sodium-stimulated transport. The accumulated results suggest that the sodium-activated glutamate transport system resides in the cytoplasmic membrane and that releasable binding protein(s) is not intimately involved in its function.

MeSH Terms
Biological Transport, Active/drug effects Carbon Radioisotopes Cell Membrane/metabolism Electron Transport/drug effects Escherichia coli/growth & development,metabolism Glutamates/metabolism Glycerol/metabolism Mutation Osmosis Proline/metabolism Protein Binding Sodium/pharmacology Stereoisomerism Tritium
Chemicals
Carbon Radioisotopes Glutamates Tritium Proline Sodium Glycerol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Miner K M
Frank L
References (27)
27 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.117.3.1093-1098.1974
Published
1974-03-00
Pages
1093-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC246588
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.117.3.1093-1098.1974 PMC Full Text View journal details
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