Proteins at the tRNA binding sites of Escherichia coli ribosomes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 1 ·1974-01-00 ·Pages 230-4

Czernilofsky AP, Collatz EE, Stöffler G, Kuechler E

Abstract

p - Nitrophenylcarbamyl - phenylalanyl-tRNA binds to ribosomes in response to poly(U) and reacts with ribosomal proteins via covalent bond formation. Ribosomal proteins of Escherichia coli were characterized by two-dimensional gel electrophoresis and by reaction with specific antibodies. The affinity label is shown to react with the 50S proteins L27, L15, L2, L16, and L14. We therefore conclude that these particular proteins are located at or near tRNA-binding sites within the 50S ribosomal subunit.

MeSH Terms

Animals Antigen-Antibody Complex Autoradiography Bacterial Proteins/analysis,metabolism Binding Sites/drug effects Carbamates Centrifugation, Density Gradient Electrophoresis, Polyacrylamide Gel Escherichia coli/cytology,metabolism Immunoassay Molecular Weight Nitrophenols Phenylalanine Protein Binding Puromycin/pharmacology RNA, Transfer/metabolism Rabbits/immunology Ribosomes/metabolism Tritium

Chemicals

Antigen-Antibody Complex Bacterial Proteins Carbamates Nitrophenols Tritium Phenylalanine Puromycin RNA, Transfer

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Czernilofsky A P

Collatz E E

Stöffler G

Kuechler E

References (23)

23 references, click to expand

A method for determining the sedimentation behavior of enzymes: application to protein mixtures.
J Biol Chem. 1961 May;236:1372-9 PMID: 13767412
Affinity label for the tRNA binding site on the Escherichia coli ribosome.
Biochim Biophys Acta. 1972 Jul 31;272(4):667-71 PMID: 4559256
Sequence differences of Escherichia coli 30S ribosomal proteins as determined by immunochemical methods.
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2283-7 PMID: 5002431
Identification of the chloramphenicol-binding protein in Escherichia coli ribosomes by partial reconstitution.
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2224-8 PMID: 4365366
Studies on the formation of transfer ribonucleic acid-ribosome complexes. XI. Antibiotic effects on phenylalanyl-oligonucleotide binding to ribosomes.
Proc Natl Acad Sci U S A. 1969 Oct;64(2):709-14 PMID: 5261043
Three-dimensional structure of yeast phenylalanine transfer RNA: folding of the polynucleotide chain.
Science. 1973 Jan 19;179(4070):285-8 PMID: 4566654
Studies on the formation of transfer ribonucleic acid-ribosome complexes. 8. Aminoacyl oligonucleotide binding to ribosomes: characteristics and requirements.
J Biol Chem. 1970 Nov 25;245(22):6208-19 PMID: 5484475
Polypeptide chain initiation: nucleotide sequences of the three ribosomal binding sites in bacteriophage R17 RNA.
Nature. 1969 Dec 6;224(5223):957-64 PMID: 5360547
Ribosomal protein neighborhoods. I. S18 and S21 as well as S5 and S8 are neighbors.
Mol Gen Genet. 1972;119(4):357-66 PMID: 4630861
Assembly mapping of 30S ribosomal proteins from E. coli.
Nature. 1970 Jun 27;226(5252):1214 PMID: 4912319
Spatial arrangement of ribosomal proteins: reaction of the Escherichia coli 30S subunit with bis-imidoesters.
Proc Natl Acad Sci U S A. 1972 May;69(5):1327-31 PMID: 4556460
Ribosomal proteins. XXV. Immunological studies on Escherichia coli ribosomal proteins.
J Mol Biol. 1971 Dec 14;62(2):407-9 PMID: 5003000
Covalent attachment of a peptidyl-transfer RNA analog to the 50S subunit of Escherichia coli ribosomes.
Proc Natl Acad Sci U S A. 1972 Apr;69(4):837-41 PMID: 4554533
Studies on the action of tetracycline and puromycin.
J Biol Chem. 1972 Jan 10;247(1):45-50 PMID: 4552926
50-S ribosomal proteins. Peptide studies on two acidic proteins, A 1 and A 2 , isolated from 50-S ribosomes of Escherichia coli.
Eur J Biochem. 1972 Jan 31;25(1):13-9 PMID: 4554103
Irreversible binding of chloramphenicol analogues to E. coli ribosomes.
FEBS Lett. 1972 Dec 1;28(2):149-152 PMID: 11946844
Position of the initiator and peptidyl sites in the E. coli ribosome.
Nature. 1970 Mar 7;225(5236):920-4 PMID: 4905902
Identification of chloramphenicol-binding protein in Escherichia coli ribosomes by affinity labeling.
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2229-33 PMID: 4599619
Chemical studies on methionyl-tRNA synthetase from Escherichia coli.
J Mol Biol. 1970 Sep 14;52(2):165-78 PMID: 4922213
Ribosomal proteins. XXXII. Comparison of several extraction methods for proteins from Escherichia coli ribosomes.
Biochimie. 1972;54(2):167-75 PMID: 4563932
Ribosomal proteins. XII. Number of proteins in small and large ribosomal subunits of Escherichia coli as determined by two-dimensional gel electrophoresis.
Proc Natl Acad Sci U S A. 1970 Nov;67(3):1276-82 PMID: 4922286
Synthesis of tRNA ureido derivatives as substrates for The invention of the ribosome peptidyl transferase center.
FEBS Lett. 1972 Aug 1;24(2):201-203 PMID: 11946672
Use of bifunctional imidoesters in the study of ribosome topography.
J Mol Biol. 1972 Feb 28;64(1):297-303 PMID: 4552483

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.1.230

Published

1974-01-00

Pages

230-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC387971

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.1.230 PMC Full Text View journal details

Analysis Services

Proteins at the tRNA binding sites of Escherichia coli ribosomes.

Contact

WeChat Official Account