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PMID: 4584813 Published · ppublish English Journal Article

Effect of rifampin on the structure and membrane attachment of the nucleoid of Escherichia coli.

Journal of bacteriology ·Vol. 116 ·No. 3 ·1973-12-00 ·Pages 1364-74

Dworsky P, Schaechter M

Abstract

Deoxyribonucleic acid (DNA) of Escherichia coli was found to be attached to the cell membrane at about 20 points. This was determined by fractionation of X-irradiated cells with the M band (magnesium-Sarkosyl crystals) technique. The number of attachment points was computed from the relationship between the amount of DNA in M bands and the number of double-strand breaks introduced by the X-ray treatment. The number of attachment points was decreased fourfold by treatment of cells with rifampin. This effect was apparently due to the action of the drug on ribonucleic acid (RNA) polymerase since the drug did not affect a mutant whose RNA polymerase is resistant to rifampin. This suggests that there may be two classes of attachment points of DNA on the membrane, some of which are removed by rifampin treatment and some which are not. Rifampin treatment also resulted in the uncondensing of isolated nucleoids and in an axial appearance of the nucleoids in ultrathin sections. The results suggest that RNA polymerase plays a role, direct or indirect, in maintaining the structure of the bacterial nucleoid and in some of its attachment to the membrane.

MeSH Terms
Carbon Radioisotopes Cell Fractionation Cell Membrane Cell Nucleus/drug effects DNA, Bacterial/analysis,radiation effects DNA-Directed RNA Polymerases/metabolism Escherichia coli/analysis,cytology,drug effects,enzymology,radiation effects Magnesium Microscopy, Electron Models, Chemical Molecular Weight Mutation Radiation Effects Rifampin/pharmacology Surface-Active Agents Tritium Viscosity
Chemicals
Carbon Radioisotopes DNA, Bacterial Surface-Active Agents Tritium DNA-Directed RNA Polymerases Magnesium Rifampin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dworsky P
Schaechter M
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32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.116.3.1364-1374.1973
Published
1973-12-00
Pages
1364-74
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC246496
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.116.3.1364-1374.1973 PMC Full Text View journal details
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