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PMID: 4584596 Published · ppublish English Journal Article

Use of combusted natural gas to cultivate the anaerobic bacterial flora from the cecum contents of mice.

Applied microbiology ·Vol. 26 ·No. 4 ·1973-10-00 ·Pages 584-8

Koopman JP, van Oeveren JP, Janssen FG

Abstract

The use of combusted natural gas as atmosphere for cultivation of the anaerobic bacterial flora from the cecum of mice is described. The combusted gas (inert gas) continuously flushes a glove box in which it is possible to cultivate a number of strict anaerobes from the cecum of mice.

MeSH Terms
Anaerobiosis Animals Bacteriological Techniques Carbon Dioxide Catalysis Cecum/microbiology Culture Media Equipment and Supplies Gases Germ-Free Life Methods Mice Mice, Inbred Strains Nitrogen Oxidation-Reduction
Chemicals
Culture Media Gases Carbon Dioxide Nitrogen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Koopman J P
van Oeveren J P
Janssen F G
References (8)
8 references, click to expand
  1. Enumeration of the oxygen sensitive bacteria usually present in the intestine of healthy mice.
    Nature. 1968 Dec 14;220(5172):1137-9 PMID: 4881378
  2. Isolation of anaerobic bacteria from human gingiva and mouse cecum by means of a simplified glove box procedure.
    Appl Microbiol. 1969 Apr;17(4):568-76 PMID: 4890748
  3. Associations of indigenous microorganisms with gastrointestinal mucosal epithelia.
    Am J Clin Nutr. 1970 Nov;23(11):1495-501 PMID: 5475367
  4. The anaerobic bacterial flora of the mouse cecum.
    J Exp Med. 1970 Aug 1;132(2):251-60 PMID: 4927597
  5. Colonization resistance of the digestive tract in conventional and antibiotic-treated mice.
    J Hyg (Lond). 1971 Sep;69(3):405-11 PMID: 4999450
  6. The intestinal flora of colonization-resistant mice.
    J Hyg (Lond). 1971 Sep;69(3):413-21 PMID: 4937854
  7. Anaerobic CO 2 cabinet for the cultivation of strict anerobes.
    Appl Microbiol. 1971 Nov;22(5):824-7 PMID: 4943587
  8. THE RUMEN BACTERIA AND PROTOZOA.
    Annu Rev Microbiol. 1964;18:131-66 PMID: 14268853
Article Info
Journal
Applied microbiology
Abbr.
Appl Microbiol
ISSN
0003-6919
Published
1973-10-00
Pages
584-8
Language
English
Region
United States
NLM ID
7605802
PMCID
PMC379853
Subset
IM
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