Isolation and characterization of phi80 transducing bacteriophage for a ribonucleic acid polymerase gene.

Journal of bacteriology ·Vol. 116 ·No. 2 ·1973-11-00 ·Pages 511-6

Konrad B, Kirschbaum J, Austin S

Abstract

A new method for isolating specialized transducing phages is described. It was used to isolate a group of phi80 transducing phages which carry various bacterial markers from the metB region of the Escherichia coli chromosome. Some of the phages selected for transduction of the supA36 marker were also shown to carry rif, a locus known to specify the beta subunit of ribonucleic acid polymerase. Expression of the prophage rif(r) gene in lysogens was demonstrated by its ability to confer rifampin resistance on part of the cellular ribonucleic acid polymerase pool.

MeSH Terms

Chromosome Mapping Chromosomes, Bacterial Coliphages/isolation & purification Culture Media DNA-Directed RNA Polymerases/metabolism Diploidy Drug Resistance, Microbial Escherichia coli/enzymology Genes Genotype Lysogeny Methionine/metabolism Mutation Phenotype Rifampin/pharmacology Transduction, Genetic

Chemicals

Culture Media Methionine DNA-Directed RNA Polymerases Rifampin

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Konrad B

Kirschbaum J

Austin S

References (15)

15 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.116.2.511-516.1973

Published

1973-11-00

Pages

511-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC285411

Subset

External Links

PubMed source DOI: 10.1128/jb.116.2.511-516.1973 PMC Full Text View journal details

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Isolation and characterization of phi80 transducing bacteriophage for a ribonucleic acid polymerase gene.

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