The mechanism of action of methionyl-tRNA synthetase. 3. Ion requirements and kinetic parameters of the ATP-PPi exchange and methionine-transfer reactions catalyzed by the native and trypsin-modified enzymes.

Acylation Adenosine Triphosphate Amino Acyl-tRNA Synthetases/metabolism Ammonium Chloride Catalysis Escherichia coli/enzymology Kinetics Magnesium Methionine Potassium Chloride Protein Binding Protein Conformation RNA, Transfer Spectrometry, Fluorescence Spermidine Spermine Structure-Activity Relationship Trypsin

Ammonium Chloride Spermine Potassium Chloride Adenosine Triphosphate RNA, Transfer Methionine Trypsin Amino Acyl-tRNA Synthetases Magnesium Spermidine