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PMID: 4579010 Published · ppublish English Journal Article

Cell envelope and shape of Escherichia coli K12.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 7 ·1973-07-00 ·Pages 2033-6

Henning U, Rehn K, Hoehn B

Abstract

Rod-shaped "ghosts" that are free of murein have been isolated from E. coli. The shape of these "ghosts" is maintained by a unit membrane soluble in sodium dodecyl sulfate. Ghosts consist of about 20-30% phospholipid (almost exclusively phosphatidylethanolamine) and 50-60% protein; a large fraction of the remaining material is lipopolysaccharide. Sodium dodecyl sulfate-gel electrophoresis reveals 4-5 different bands corresponding to molecular weights between 10,000 and 40,000. Treatment of ghosts with Pronase reduces this number to 3, and the rod shape still is not lost. Results of treatment of ghosts with a crude extract from Dictyostelium discoideum have supplied tentative evidence that at least one of these proteins is involved in the maintenance of rod shape. It does not appear too unlikely that these polypeptide chains are the final products of the genetic information specifying cellular shape.

MeSH Terms
Bacterial Proteins/analysis Bacteriolysis Cell Fractionation Cell Membrane/analysis Centrifugation Electrophoresis Escherichia coli/analysis,cytology Lipoproteins/analysis Microscopy, Electron Muramidase Peptidoglycan/analysis Phosphatidylethanolamines/analysis Phospholipids/analysis Pronase Sodium Dodecyl Sulfate
Chemicals
Bacterial Proteins Lipoproteins Peptidoglycan Phosphatidylethanolamines Phospholipids Sodium Dodecyl Sulfate Muramidase Pronase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Henning U
Rehn K
Hoehn B
References (20)
20 references, click to expand
  1. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  2. Effects of oleate starvation in a fatty acid auxotroph of Escherichia coli K-12.
    J Bacteriol. 1969 May;98(2):784-96 PMID: 4891268
  3. The covalent murein-lipoprotein structure of the Escherichia coli cell wall. The attachment site of the lipoprotein on the murein.
    Eur J Biochem. 1970 Apr;13(2):336-46 PMID: 4245367
  4. Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X-100.
    J Bacteriol. 1971 Oct;108(1):545-52 PMID: 4941569
  5. The fluid mosaic model of the structure of cell membranes.
    Science. 1972 Feb 18;175(4023):720-31 PMID: 4333397
  6. Repetitive sequences in the murein-lipoprotein of the cell wall of Escherichia coli.
    Proc Natl Acad Sci U S A. 1972 Apr;69(4):970-4 PMID: 4260278
  7. Cell envelope and shape of Escherichia coli K12. Properties of a temperature-sensitive rod mutant.
    Eur J Biochem. 1972 Apr 24;26(4):570-86 PMID: 4554573
  8. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.
    J Biol Chem. 1972 Jun 25;247(12):3962-72 PMID: 4555955
  9. Organization of proteins in the native and reformed outer membrane of Escherichia coli.
    Biochim Biophys Acta. 1972 Aug 9;274(2):478-88 PMID: 4558851
  10. Degradation of the murein-lipoprotein complex of Escherichia coli cell walls by Dictyostelium amoebae.
    Eur J Biochem. 1972 May;27(1):116-25 PMID: 4261814
  11. Purification of bacterial membrane proteins. The use of guanidinium thiocyanate and urea.
    J Membr Biol. 1972;10(2):137-52 PMID: 4592401
  12. Cell wall and cytoplasmic membrane of Escherichia coli.
    J Biophys Biochem Cytol. 1958 May 25;4(3):323-6 PMID: 13549505
  13. The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. I. Identification.
    J Biol Chem. 1959 Apr;234(4):705-9 PMID: 13654246
  14. Physical principles in the construction of regular viruses.
    Cold Spring Harb Symp Quant Biol. 1962;27:1-24 PMID: 14019094
  15. METABOLISM AND FUNCTION OF BACTERIAL LIPIDS. I. METABOLISM OF PHOSPHOLIPIDS IN ESCHERICHIA COLI B.
    J Biol Chem. 1963 Sep;238:2919-22 PMID: 14081903
  16. BAGSHAPED MACROMOLECULES--A NEW OUTLOOK ON BACTERIAL CELL WALLS.
    Adv Enzymol Relat Areas Mol Biol. 1964;26:193-232 PMID: 14150645
  17. THE LOCATION OF THE MUCOPEPTIDE IN SECTIONS OF THE CELL WALL OF ESCHERICHIA COLI AND OTHER GRAM-NEGATIVE BACTERIA.
    Can J Microbiol. 1965 Jun;11:547-60 PMID: 14346132
  18. Production and ultrastructure of lysozyme and ethylenediaminetetraacetate-lysozyme spheroplasts of Escherichia coli.
    J Bacteriol. 1967 Jan;93(1):427-37 PMID: 4960155
  19. Ultrastructure of the cell wall of Escherichia coli and chemical nature of its constituent layers.
    J Ultrastruct Res. 1967 Jul;19(1):45-83 PMID: 4961452
  20. Chemical characterization, spatial distribution and function of a lipoprotein (murein-lipoprotein) of the E. coli cell wall. The specific effect of trypsin on the membrane structure.
    Eur J Biochem. 1969 Oct;10(3):426-38 PMID: 4899922
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.7.2033
Published
1973-07-00
Pages
2033-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433659
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.7.2033 PMC Full Text View journal details
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