In vitro repression of transcription of the tryptophan operon by trp repressor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 7 ·1973-07-00 ·Pages 1990-4

Shimizu Y, Shimizu N, Hayashi M

Abstract

The in vitro repression of transcription of the tryptophan operon by the trp repressor from Escherichia coli was studied. By measuring the inhibitory effect for trp-specific RNA synthesis in an in vitro transcription system directed by DNA of trp-transducing phage, we have detected and concentrated a trp repressor in an eluate of a Phi80 ptED native DNA-cellulose column. The repression of transcription of trp operon required the addition of L-tryptophan in the system, and when several tryptophan analogues were added, the repression or derepression was similar to that observed in vivo. The repressor fraction was separated from the majority of tryptophanyl-tRNA synthetase activity by Bio-gel P60 column chromatography.

MeSH Terms

Acrylates/pharmacology Amino Acyl-tRNA Synthetases/metabolism Cellulose Chromatography, Affinity Coliphages DNA, Viral Enzyme Repression Escherichia coli/enzymology,metabolism Genes, Regulator Indoles/pharmacology Nucleic Acid Hybridization Operon RNA, Bacterial/analysis,biosynthesis Templates, Genetic Transcription, Genetic/drug effects Transduction, Genetic Tritium Tryptophan/biosynthesis,pharmacology

Chemicals

Acrylates DNA, Viral Indoles RNA, Bacterial Tritium Tryptophan Cellulose Amino Acyl-tRNA Synthetases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Shimizu Y

Shimizu N

Hayashi M

References (26)

26 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.7.1990

Published

1973-07-00

Pages

1990-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433649

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.7.1990 PMC Full Text View journal details

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In vitro repression of transcription of the tryptophan operon by trp repressor.

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