Studies on the conversion of proinsulin to insulin. 3. Studies in vitro with a crude secretion granule fraction isolated from rat islets of Langerhans.

Animals Arginine/metabolism Carboxypeptidases/metabolism Centrifugation, Density Gradient Chromatography, Gel Cytoplasmic Granules/metabolism Electrophoresis, Polyacrylamide Gel Esters/metabolism Female Insulin/biosynthesis Islets of Langerhans/cytology,enzymology,metabolism Leucine/metabolism Peptides/isolation & purification,metabolism Proinsulin/metabolism Protease Inhibitors Rats Structure-Activity Relationship Time Factors Tosyl Compounds/metabolism Tritium Trypsin/metabolism

Esters Insulin Peptides Protease Inhibitors Tosyl Compounds Tritium Proinsulin Arginine Carboxypeptidases Trypsin Leucine