Nucleotide sequence divergence in the -chain-structural genes of tryptophan synthetase from Escherichia coli, Salmonella typhimurium, and Aerobacter aerogenes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 4 ·1973-04-00 ·Pages 1112-6

Li SL, Denney RM, Yanofsky C

Abstract

Two different estimates were obtained for the extent of nucleotide sequence divergence in the structural genes of the tryptophan synthetase alpha-chains of Escherichia coli, Salmonella typhimurium, and Aerobacter aerogenes. One estimate was based on comparisons of the amino acid sequences of the respective alpha chains. The other was derived from measurements of the thermal stability of RNA-DNA hybrids formed with phage DNA carrying the alpha-chain structural gene of E. coli and labeled messenger RNA from the three bacterial species. Comparison of the two estimates suggests that during the course of evolution synonymous codon changes have accumulated in the alpha-chain-structural genes.

MeSH Terms

Amino Acid Sequence Base Sequence Coliphages DNA, Bacterial/analysis DNA, Viral/metabolism Enterobacter/enzymology Escherichia coli/enzymology Genes Genetic Code Hydro-Lyases/biosynthesis Nucleic Acid Hybridization RNA, Bacterial/metabolism RNA, Messenger/metabolism Salmonella typhimurium/enzymology Species Specificity Tryptophan Synthase/biosynthesis

Chemicals

DNA, Bacterial DNA, Viral RNA, Bacterial RNA, Messenger Hydro-Lyases Tryptophan Synthase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Li S L

Denney R M

Yanofsky C

References (14)

14 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.4.1112

Published

1973-04-00

Pages

1112-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433437

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.4.1112 PMC Full Text View journal details

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Nucleotide sequence divergence in the -chain-structural genes of tryptophan synthetase from Escherichia coli, Salmonella typhimurium, and Aerobacter aerogenes.

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