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PMID: 4576018 Published · ppublish English Journal Article

Mapping of Escherichia coli ribosomal components involved in peptidyl transferase activity.

Sonenberg N, Wilchek M, Zamir A

Abstract

The method of affinity labeling has been used to identify protein components of 50S ribosomal subunits involved in peptidyl transferase activity. E. coli 50S ribosomal subunits were mapped by reaction with the N-bromoacetyl analog of chloramphenicol, an antibiotic known to interact specifically with the active center of the enzyme. The synthetic analog competes with chloramphenicol in binding to 50S ribosomal subunits and inhibits peptidyl transferase activity. It attaches covalently to the ribosome under appropriate conditions and causes an irreversible loss in peptidyl transferase activity. The reagent specifically alkylates cysteine residues of proteins L2 and L27.

MeSH Terms
Acetamides/metabolism Acyltransferases/antagonists & inhibitors,metabolism Alkylation Anti-Bacterial Agents/metabolism Bacterial Proteins/analysis,biosynthesis Binding, Competitive Bromine Carbon Isotopes Chloramphenicol/metabolism Cysteine/metabolism Escherichia coli/enzymology Ethylmaleimide/metabolism Methionine/metabolism Methods Peptide Elongation Factors/antagonists & inhibitors,metabolism Peptides Phenethylamines/metabolism Phenylalanine/metabolism Ribosomes/enzymology Succinimides/metabolism
Chemicals
Acetamides Anti-Bacterial Agents Bacterial Proteins Carbon Isotopes Peptide Elongation Factors Peptides Phenethylamines Succinimides Phenylalanine Chloramphenicol Methionine Acyltransferases Cysteine Ethylmaleimide Bromine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sonenberg N
Wilchek M
Zamir A
References (19)
19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Published
1973-05-00
Pages
1423-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433511
Subset
IM
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