Caloric catastrophe.

Biophysical journal ·Vol. 13 ·No. 2 ·1973-02-00 ·Pages 167-78

Minkoff L, Damadian R

Abstract

Six solutes known to be actively transported by bacteria were studied with the cell in a "minimum energy" state to determine if sufficient energy were available from cellular stores of ATP to supply the energy necessary to run postulated membrane-situated "pumps." Steady-state cellular concentrations of potassium, calcium, magnesium, leucine, glycine, and alpha-methyl glucoside were determined together with tracer fluxes, oxygen consumption, ATP turnover, and the P:O ratio. From these measurements, it was calculated that the energy supply, 4.20 cal/340 min-g dry wt, fell far short of the energy necessary (28.28 cal/340 min-g dry wt), by classical membrane theory, to operate "pumps."

MeSH Terms

Adenosine Triphosphate/metabolism Biological Transport Calcium/metabolism Cell Membrane Permeability Escherichia coli/metabolism Glucose/metabolism Glycine/metabolism Glycosides/metabolism Kinetics Leucine/metabolism Magnesium/metabolism Mathematics Oxidative Phosphorylation Oxygen Consumption Phosphorus Isotopes Potassium/metabolism

Chemicals

Glycosides Phosphorus Isotopes Adenosine Triphosphate Leucine Magnesium Glucose Potassium Calcium Glycine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Minkoff L

Damadian R

References (17)

17 references, click to expand

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Article Info

Journal

Biophysical journal

Abbr.

Biophys J

ISSN

0006-3495

DOI

10.1016/S0006-3495(73)85977-6

Published

1973-02-00

Pages

167-78

Language

English

Region

United States

NLM ID

0370626

PMCID

PMC1484216

Subset

Corrections

CommentIn

PMID: 19211021

External Links

PubMed source DOI: 10.1016/S0006-3495(73)85977-6 PMC Full Text View journal details

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