Physical assay of competence for specific mating-pair formation in Escherichia coli.

Journal of bacteriology ·Vol. 114 ·No. 1 ·1973-04-00 ·Pages 144-51

Walmsley RH

Abstract

The fractions of Escherichia coli female and male bacteria that are competent to form specific mating pairs at any time during normal exponential growth have been measured by a physical assay. The competencies of a Hayes- and a Cavali-type donor were 35 and 50%, respectively, in agreement with piliation assays reported by others. The competencies of two recipient strains, PA309 and P678, were found to be 33 and 29%, respectively.

MeSH Terms

Crosses, Genetic Culture Media Escherichia coli/cytology,growth & development Female Kinetics Male Methods Statistics as Topic

Chemicals

Culture Media

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Walmsley R H

References (15)

15 references, click to expand

The structure, function, synthesis and genetic control of bacterial pili and a molecular model for DNA and RNA transport in gram negative bacteria.
Trans N Y Acad Sci. 1965 Jun;27(8):1003-54 PMID: 5318403
The sex hair of E. coli as sensory fiber, conjugation tube, or mating arm?
Biochem Biophys Res Commun. 1967 Jun 23;27(6):674-80 PMID: 4862405
Revised linkage map of Escherichia coli.
Bacteriol Rev. 1967 Dec;31(4):332-53 PMID: 4865540
Inhibition of bacterial conjugation by ribonucleic acid and deoxyribonucleic acid male-specific bacteriophages.
J Bacteriol. 1968 Feb;95(2):314-26 PMID: 4867736
Kinetics of recombinant formation in synchronized cultures of Escherichia coli.
J Bacteriol. 1968 Jun;95(6):2442-3 PMID: 4876139
Mechanism of conjugation and recombination in bacteria. VI. Receptibility of F- cells for donor genophore during the cell-life cycle.
Acta Microbiol Pol A. 1969;1(2):79-85 PMID: 4897599
Bacterial conjugation.
Annu Rev Microbiol. 1969;23:69-136 PMID: 4899081
Early stages of conjugation in Escherichia coli.
J Bacteriol. 1969 Nov;100(2):1091-104 PMID: 4902383
Effects of cell wall polysaccharide on the mating ability of Salmonella typhimurium.
Nature. 1970 Jan 3;225(5227):70-1 PMID: 4903100
Role of pili in bacterial conjugation.
J Bacteriol. 1970 Jun;102(3):648-54 PMID: 4914070
Ampicillin-resistant mutants of Escherichia coli K-12 with lipopolysaccharide alterations affecting mating ability and susceptibility to sex-specific bacteriophages.
J Bacteriol. 1971 Aug;107(2):420-32 PMID: 4939761
[Sexuality of bacteria].
Boll Ist Sieroter Milan. 1950 Sep-Oct;29(9-10):281-9 PMID: 14801088
The mechanism of genetic recombination in Escherichia coli.
Cold Spring Harb Symp Quant Biol. 1953;18:75-93 PMID: 13168972
Recombination and segregation in Escherichia coli.
Cold Spring Harb Symp Quant Biol. 1958;23:47-58 PMID: 13635540
The genetic control of colicinogenic factors E2, I and V.
J Gen Microbiol. 1962 Nov;29:473-84 PMID: 14026126

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.114.1.144-151.1973

Published

1973-04-00

Pages

144-51

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC251750

Subset

External Links

PubMed source DOI: 10.1128/jb.114.1.144-151.1973 PMC Full Text View journal details

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Physical assay of competence for specific mating-pair formation in Escherichia coli.

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