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PMID: 4572705 Published · ppublish English Journal Article

Physiology of Escherichia coli K-12 during conjugation: altered recipient cell functions associated with lethal zygosis.

Journal of bacteriology ·Vol. 114 ·No. 1 ·1973-04-00 ·Pages 11-7

Skurray RA, Reeves P

Abstract

The number of viable F(-) cells decreases when Escherichia coli recipient cells are mixed with an excess of Hfr cells. Evidence is presented showing that lethal zygosis was accompanied by changes in the physiology of the recipient cells, including (i) inhibition of deoxyribonucleic acid synthesis, (ii) inhibition of beta-galactosidase induction, (iii) altered transport and accumulation of galactosides, and (iv) leakage of beta-galactosidase into the supernatant fluid. The results are discussed in terms of possible conjugation-associated changes that, at high Hfr to F(-) ratios, lead to lethal zygosis.

MeSH Terms
Autoradiography Biological Transport Carbon Isotopes/metabolism Conjugation, Genetic Culture Media DNA, Bacterial/biosynthesis Enzyme Induction Escherichia coli/enzymology,metabolism Galactosidases/biosynthesis,metabolism Genes, Lethal Genotype Hydrolysis Membrane Transport Proteins/metabolism Mutation Selection, Genetic Thymidine/metabolism Thymine/metabolism Time Factors Tritium
Chemicals
Carbon Isotopes Culture Media DNA, Bacterial Membrane Transport Proteins Tritium Galactosidases Thymine Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Skurray R A
Reeves P
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27 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.114.1.11-17.1973
Published
1973-04-00
Pages
11-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC251734
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.114.1.11-17.1973 PMC Full Text View journal details
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